KO Validated Antibodies 1

Anti-NT5E / CD73 Antibody (CAB2029)[KO Validated]

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SKU:
CAB2029
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NT5E / CD73 Antibody [KO Validated]
Antibody SKU:CAB2029
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human NT5E / CD73 (NP_002517.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:DU145, Mouse liver, Mouse lung, Rat brain, Rat liver, HCT116
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human NT5E / CD73 (NP_002517.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LKIE FDER GNVI SSHG NPIL LNSS IPED PSIK ADIN KWRI KLDN YSTQ ELGK TIVY LDGS SQSC RFRE CNMG NLIC DAMI NNNL RHTD EMFW NHVS MCIL NGGG IRSP IDER NNGT ITWE NLAA VLPF GGTF DLVQ LKGS TLKK AFEH SVHR YGQS TGEF LQVG GIHV VYDL SRKP GDRV VKLD VLCT KCRV PSYD PLKM DEVY KVIL PNFL ANGG DGFQ MIKD ELLR HDSG DQDI NVVS TYIS KMKV IYPA VEGR IKFS T
Gene ID:4907
Uniprot:P21589
Cellular Location:Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:57kDa/63kDa
Observed MW:69kDa/75kDa
Synonyms:NT5E, CALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT
Background:The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function:5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries

NCBI Summary:The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
UniProt Code:P21589
NCBI GenInfo Identifier:112825
NCBI Gene ID:4907
NCBI Accession:P21589.1
UniProt Related Accession:P21589
Molecular Weight:
NCBI Full Name:5'-nucleotidase
NCBI Synonym Full Names:5'-nucleotidase ecto
NCBI Official Symbol:NT5E  
NCBI Official Synonym Symbols:NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA  
NCBI Protein Information:5'-nucleotidase
UniProt Protein Name:5'-nucleotidase
UniProt Synonym Protein Names:Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name:NT5E  
UniProt Entry Name:5NTD_HUMAN
Anti-NT5E / CD73 Antibody [KO Validated] (CAB2029)Western blot analysis of extracts of various cell lines, using NT5E / CD73 antibody (CAB2029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-NT5E / CD73 Antibody [KO Validated] (CAB2029)Western blot analysis of extracts from normal (control) and NT5E / CD73 Rabbit pAb knockout (KO) HCT116 cells, using NT5E / CD73 Rabbit pAb antibody (CAB2029) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-NT5E / CD73 Antibody [KO Validated] (CAB2029)Immunohistochemistry of paraffin-embedded rat spleen using NT5E/CD73 antibody (CAB2029) at dilution of 1:200 (40x lens).
Anti-NT5E / CD73 Antibody [KO Validated] (CAB2029)Immunohistochemistry of paraffin-embedded human placenta using NT5E/CD73 antibody (CAB2029) at dilution of 1:200 (40x lens).
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