Metabolism Antibodies 1

Anti-NT5C3A Antibody (CAB14127)

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SKU:
CAB14127
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-NT5C3A Antibody
Antibody SKU:CAB14127
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-336 of human NT5C3A (NP_001002010.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-336 of human NT5C3A (NP_001002010.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TLKR KTGR KTKI IEMM PEFQ KSSV RIKN PTRV EEII CGLI KGGA AKLQ IITD FDMT LSRF SYKG KRCP TCHN IIDN CKLV TDEC RKKL LQLK EKYY AIEV DPVL TVEE KYPY MVEW YTKS HGLL VQQA LPKA KLKE IVAE SDVM LKEG YENF FDKL QQHS IPVF IFSA GIGD VLEE VIRQ AGVY HPNV KVVS NFMD FDET GVLK GFKG ELIH VFNK HDGA LRNT EYFN QLKD NSNI ILLG DSQG DLRM ADGV ANVE HILK IGYL NDRV DELL EKYM DSYD IVLV QDES LEVA NSIL QKIL
Gene ID:51251
Uniprot:Q9H0P0
Cellular Location:Cytoplasm, Endoplasmic reticulum
Calculated MW:32kDa/33kDa/37kDa
Observed MW:38kDa
Synonyms:NT5C3A, NT5C3, P5N-1, PN-I, POMP, PSN1, UMPH, UMPH1, cN-III, hUMP1, p36
Background:This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4.
UniProt Protein Function:NT5C3A: Can act both as nucleotidase and as phosphotransferase. Defects in NT5C3 are the cause of P5N deficiency (P5ND); also called hemolytic anemia due to P5N deficiency or hemolytic anemia due to UMPH1 deficiency. P5ND is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte. It is implicated in the anemia of lead poisoning and is possibly associated with learning difficulties. Belongs to the pyrimidine 5'-nucleotidase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); Transferase; EC 3.1.3.5; Endoplasmic reticulum; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum

Molecular Function:2'-phosphotransferase activity; 5'-nucleotidase activity

Biological Process: pyrimidine nucleoside catabolic process

Disease: Uridine 5-prime Monophosphate Hydrolase Deficiency, Hemolytic Anemia Due To

NCBI Summary:This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012]
UniProt Code:Q9H0P0
NCBI GenInfo Identifier:117949804
NCBI Gene ID:51251
NCBI Accession:Q9H0P0.3
UniProt Secondary Accession:Q9H0P0,Q6IPZ1, Q6NXS6, Q7L3G6, Q9P0P5, Q9UC42, Q9UC43 Q9UC44, Q9UC45, A8K253, B2RAA5, B8ZZC4,
UniProt Related Accession:Q9H0P0
Molecular Weight:32,559 Da
NCBI Full Name:Cytosolic 5'-nucleotidase 3A
NCBI Synonym Full Names:5'-nucleotidase, cytosolic IIIA
NCBI Official Symbol:NT5C3A  
NCBI Official Synonym Symbols:p36; PN-I; POMP; PSN1; UMPH; NT5C3; P5N-1; UMPH1; hUMP1; P5'N-1; cN-III  
NCBI Protein Information:cytosolic 5'-nucleotidase 3A
UniProt Protein Name:Cytosolic 5'-nucleotidase 3A
UniProt Synonym Protein Names:7-methylguanosine phosphate-specific 5'-nucleotidase
Protein Family:Cytosolic 5'-nucleotidase
UniProt Gene Name:NT5C3A  
UniProt Entry Name:5NT3A_HUMAN
Anti-NT5C3A Antibody (CAB14127)Western blot analysis of extracts of mouse brain, using NT5C3A antibody (CAB14127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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