Cell Biology Antibodies 11

Anti-NSUN6 Antibody (CAB7205)

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SKU:
CAB7205
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NSUN6 Antibody
Antibody SKU:CAB7205
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_872349.1).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Jurkat, HepG2, HeLa, 293T, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_872349.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NLPS ALVS HVLN PQPG EKIL DLCA APGG KTTH IAAL MHDQ GEVI ALDK IFNK VEKI KQNA LLLG LNSI RAFC FDGT KAVK LDMV EDTE GEPP FLPE SFDR ILLD APCS GMGQ RPNM ACTW SVKE VASY QPLQ RKLF TAAV QLLK PEGV LVYS TCTI TLAE NEEQ VAWA LTKF PCLQ LQPQ EPQI GGEG MRGA GLSC EQLK QLQR FDPS AVPL PDTD MDSL REAR REDM LRLA NKDS IGFF IAKF VKCK ST
Gene ID:221078
Uniprot:Q8TEA1
Cellular Location:
Calculated MW:51kDa
Observed MW:52kDa
Synonyms:NSUN6, 4933414E04Rik, ARL5B-AS1, NOPD1
Background:
UniProt Protein Function:NSUN6: May have S-adenosyl-L-methionine-dependent methyl- transferase activity. Belongs to the methyltransferase superfamily. RsmB/NOP family.
UniProt Protein Details:

Protein type:Methyltransferase; Methyltransferase, protein arginine, predicted; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 10p12.31

Molecular Function:methyltransferase activity; RNA binding

Biological Process: methylation

UniProt Code:Q8TEA1
NCBI GenInfo Identifier:74751440
NCBI Gene ID:221078
NCBI Accession:Q8TEA1.1
UniProt Secondary Accession:Q8TEA1,B0YJ54,
UniProt Related Accession:Q8TEA1
Molecular Weight:469
NCBI Full Name:Putative methyltransferase NSUN6
NCBI Synonym Full Names:NOP2/Sun domain family, member 6
NCBI Official Symbol:NSUN6  
NCBI Official Synonym Symbols:NOPD1; ARL5B-AS1; 4933414E04Rik  
NCBI Protein Information:putative methyltransferase NSUN6; ARL5B antisense RNA 1; NOL1/NOP2/Sun domain family, member 6; NOL1/NOP2/Sun and PUA domain-containing protein 1; nucleolar protein (NOL1/NOP2/sun) and PUA domains 1
UniProt Protein Name:Putative methyltransferase NSUN6
UniProt Synonym Protein Names:NOL1/NOP2/Sun and PUA domain-containing protein 1; NOL1/NOP2/Sun domain family member 6
Protein Family:Putative methyltransferase
UniProt Gene Name:NSUN6  
UniProt Entry Name:NSUN6_HUMAN
Anti-NSUN6 Antibody (CAB7205)Western blot analysis of extracts of various cell lines, using NSUN6 antibody (CAB7205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
Anti-NSUN6 Antibody (CAB7205)Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug NSUN6 antibody (CAB7205). Western blot was performed from the immunoprecipitate using NSUN6 antibody (CAB7205) at a dilition of 1:1000.
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