Cell Biology Antibodies 5

Anti-NSUN4 Antibody (CAB14983)

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SKU:
CAB14983
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NSUN4 Antibody
Antibody SKU:CAB14983
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-290 of human NSUN4 (NP_950245.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, HT-29, HeLa, HepG2, Mouse brain, Mouse testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-290 of human NSUN4 (NP_950245.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RYKK KWAA TEPK FPAV RLAL QNFD MTYS VQFG DLWP SIRV SLLS EQKY GALV NNFA AWDH VSAK LEQL SAKD FVNE AISH WELQ SEGG QSAA PSPA SWAC SPNL RCFT FDRG DISR FPPA RPGS LGVM EYYL MDAA SLLP VLAL GLQP GDIV LDLC AAPG GKTL ALLQ TGCC RNLA ANDL SPSR IARL QKIL HSYV PEEI RDGN QVRV TSWD GRKW GELE GDTY DRVL VDVP CTTD RHSL HEEE NNIF KRSR KKER QILP VLQV Q
Gene ID:387338
Uniprot:Q96CB9
Cellular Location:Mitochondrion
Calculated MW:16kDa/21kDa/37kDa/43kDa
Observed MW:43kDa
Synonyms:NSUN4, SHTAP
Background:
UniProt Protein Function:Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.
UniProt Code:Q96CB9
NCBI GenInfo Identifier:371123153
NCBI Gene ID:387338
NCBI Accession:NP_001243056.1
UniProt Secondary Accession:Q96CB9,Q5TDF7, Q96AN8, Q9HAJ8, A8K6S6, B3KQ50, B4DHA4
UniProt Related Accession:Q96CB9
Molecular Weight:43 kDa
NCBI Full Name:5-methylcytosine rRNA methyltransferase NSUN4 isoform b
NCBI Synonym Full Names:NOP2/Sun RNA methyltransferase family member 4
NCBI Official Symbol:NSUN4  
NCBI Official Synonym Symbols:SHTAP  
NCBI Protein Information:5-methylcytosine rRNA methyltransferase NSUN4
UniProt Protein Name:5-methylcytosine rRNA methyltransferase NSUN4
UniProt Synonym Protein Names:NOL1/NOP2/Sun domain family member 4
Protein Family:Sperm head and tail associated protein
UniProt Gene Name:NSUN4  
Anti-NSUN4 Antibody (CAB14983)Western blot analysis of extracts of various cell lines, using NSUN4 antibody (CAB14983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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