Cell Biology Antibodies 3

Anti-NSUN3 Antibody (CAB12892)

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SKU:
CAB12892
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NSUN3 Antibody
Antibody SKU:CAB12892
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NSUN3 (NP_071355.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, Mouse thymus, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NSUN3 (NP_071355.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLTQ LKAK SEGK LAKQ ICKV VLDH FEKQ YSKE LGDA WNTV REIL TSPS CWQY AVLL NRFN YPFE LEKD LHLK GYHT LSQG SLPN YPKS VKCY LSRT PGRI PSER HQIG NLKK YYLL NAAS
Gene ID:63899
Uniprot:Q9H649
Cellular Location:
Calculated MW:38kDa
Observed MW:38kDa
Synonyms:NSUN3, MST077, MSTP077
Background:
UniProt Protein Function:NSUN3: May have S-adenosyl-L-methionine-dependent methyl- transferase activity. Belongs to the methyltransferase superfamily. RsmB/NOP family.
UniProt Protein Details:

Protein type:EC 2.1.1.-; Methyltransferase

Chromosomal Location of Human Ortholog: 3q11.2

Cellular Component: mitochondrial large ribosomal subunit; mitochondrial matrix; mitochondrion

Molecular Function:methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity

Biological Process: rRNA methylation

UniProt Code:Q9H649
NCBI GenInfo Identifier:74733593
NCBI Gene ID:63899
NCBI Accession:Q9H649.1
UniProt Secondary Accession:Q9H649,Q6PG41, Q8IXG9, Q9H6M2,
UniProt Related Accession:Q9H649
Molecular Weight:38kDa
NCBI Full Name:tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
NCBI Synonym Full Names:NOP2/Sun RNA methyltransferase family member 3
NCBI Official Symbol:NSUN3  
NCBI Official Synonym Symbols:MST077; MSTP077  
NCBI Protein Information:tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
UniProt Protein Name:tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
UniProt Synonym Protein Names:NOL1/NOP2/Sun domain family member 3
Protein Family:Putative methyltransferase
UniProt Gene Name:NSUN3  
Anti-NSUN3 Antibody (CAB12892)Western blot analysis of extracts of various cell lines, using NSUN3 antibody (CAB12892) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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