Epigenetics & Nuclear Signaling Antibodies 2

Anti-NSMCE1 Antibody (CAB15966)

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SKU:
CAB15966
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NSMCE1 Antibody
Antibody SKU:CAB15966
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 7-266 of human NSMCE1 (NP_659547.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:BxPC-3, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 7-266 of human NSMCE1 (NP_659547.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RMGV MTDV HRRF LQLL MTHG VLEE WDVK RLQT HCYK VHDR NATV DKLE DFIN NINS VLES LYIE IKRG VTED DGRP IYAL VNLA TTSI SKMA TDFA ENEL DLFR KALE LIID SETG FASS TNIL NLVD QLKG KKMR KKEA EQVL QKFV QNKW LIEK EGEF TLHG RAIL EMEQ YIRE TYPD AVKI CNIC HSLL IQGQ SCET CGIR MHLP CVAK YFQS NAEP RCPH CNDY WPHE IPKV FDPE KERE SGVL KSNK KSLR SRQH
Gene ID:197370
Uniprot:Q8WV22
Cellular Location:Chromosome, Nucleus, telomere
Calculated MW:30kDa
Observed MW:31kDa
Synonyms:NSMCE1, NSE1, NSE1 homolog
Background:
UniProt Protein Function:NSMCE1: Component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT- associated PML bodies (APBs). Has in vitro ubiquitin ligase activity in presence of NDNL2. Is involved in positive regulation of response to DNA damage stimulus. Belongs to the NSE1 family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function:protein binding; ubiquitin-protein ligase activity

Biological Process: double-strand break repair via homologous recombination; postreplication repair; protein sumoylation

UniProt Code:Q8WV22
NCBI GenInfo Identifier:209572785
NCBI Gene ID:197370
NCBI Accession:Q8WV22.5
UniProt Secondary Accession:Q8WV22,Q9P045, Q9P049, D3DWF6,
UniProt Related Accession:Q8WV22
Molecular Weight:30,855 Da
NCBI Full Name:Non-structural maintenance of chromosomes element 1 homolog
NCBI Synonym Full Names:NSE1 homolog, SMC5-SMC6 complex component
NCBI Official Symbol:NSMCE1  
NCBI Official Synonym Symbols:NSE1  
NCBI Protein Information:non-structural maintenance of chromosomes element 1 homolog
UniProt Protein Name:Non-structural maintenance of chromosomes element 1 homolog
UniProt Gene Name:NSMCE1  
UniProt Entry Name:NSE1_HUMAN
Anti-NSMCE1 Antibody (CAB15966)Western blot analysis of extracts of various cell lines, using NSMCE1 antibody (CAB15966) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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