Cell Biology Antibodies 5

Anti-NSA2 Antibody (CAB14475)

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SKU:
CAB14475
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NSA2 Antibody
Antibody SKU:CAB14475
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human NSA2 (NP_055701.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, Mouse heart, Mouse kidney, Mouse skeletal muscle, Rat heart, Rat kidney, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human NSA2 (NP_055701.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPQN EYIE LHRK RYGY RLDY HEKK RKKE SREA HERS KKAK KMIG LKAK LYHK QRHA EKIQ MKKT IKMH EKRN TKQK NDEK TPQG AVPA YLLD REGQ SRAK VLSN MIKQ KRKE KAGK WEVP LPKV RAQG ETEV LKVI RTGK RKKK AWKR MVTK VCFV GDGF TRKP PKYE RFIR PMGL RFKK AHVT HPEL KATF CLPI LGVK KNPS SPLY TTLG VITK GTVI EVNV SELG LVTQ GGKV IWGK YAQV TNNP ENDG CINA VLLV
Gene ID:10412
Uniprot:O95478
Cellular Location:Nucleus, nucleolus
Calculated MW:30kDa
Observed MW:37kDa
Synonyms:NSA2, CDK105, HCL-G1, HCLG1, HUSSY-29, HUSSY29, TINP1
Background:This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified.
UniProt Protein Function:Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles.
NCBI Summary:This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified. [provided by RefSeq, Nov 2012]
UniProt Code:O95478
NCBI GenInfo Identifier:410651502
NCBI Gene ID:10412
NCBI Accession:NP_001258594.1
UniProt Related Accession:O95478
Molecular Weight:
NCBI Full Name:ribosome biogenesis protein NSA2 homolog isoform 2
NCBI Synonym Full Names:NSA2, ribosome biogenesis homolog
NCBI Official Symbol:NSA2  
NCBI Official Synonym Symbols:HCLG1; TINP1; CDK105; HCL-G1; HUSSY29; HUSSY-29  
NCBI Protein Information:ribosome biogenesis protein NSA2 homolog
UniProt Protein Name:Ribosome biogenesis protein NSA2 homolog
UniProt Synonym Protein Names:Hairy cell leukemia protein 1; TGF-beta-inducible nuclear protein 1
Protein Family:Ribosome biogenesis protein
UniProt Gene Name:NSA2  
Anti-NSA2 Antibody (CAB14475)Western blot analysis of extracts of various cell lines, using NSA2 antibody (CAB14475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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