Cardiovascular Antibodies

Anti-NRP1 Antibody (CAB16697)

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SKU:
CAB16697
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-NRP1 Antibody
Antibody SKU:CAB16697
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-250 of human NRP1 (NP_001019800.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:3000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, A-549, U-87MG, 293T, mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-250 of human NRP1 (NP_001019800.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AFRN DKCG DTIK IESP GYLT SPGY PHSY HPSE KCEW LIQA PDPY QRIM INFN PHFD LEDR DCKY DYVE VFDG ENEN GHFR GKFC GKIA PPPV VSSG PFLF IKFV SDYE THGA GFSI RYEI FKRG PECS QNYT TPSG VIKS PGFP EKYP NSLE CTYI VFAP KMSE IILE FESF DLEP DSNP PGGM FCRY DRLE IWDG FPDV GPHI GRYC GQKT PGRI RSSS GILS MVFY TD
Gene ID:8829
Uniprot:O14786
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:68kDa/71kDa/103kDa
Observed MW:103kDa
Synonyms:BDCA4, CD304, NP1, NRP, VEGF165R, Neuropilin-1, NRP1
Background:This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
UniProt Protein Function:NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 10p12

Cellular Component: axon; cytoplasmic vesicle; cytosol; early endosome; extracellular space; focal adhesion; plasma membrane; receptor complex

Molecular Function:coreceptor activity; growth factor binding; protein binding; vascular endothelial growth factor receptor activity

Biological Process: angiogenesis; artery morphogenesis; axon extension involved in axon guidance; cell migration during sprouting angiogenesis; cell-cell signaling; hepatocyte growth factor receptor signaling pathway; nerve development; neuron migration; organ morphogenesis; patterning of blood vessels; platelet-derived growth factor receptor signaling pathway; positive chemotaxis; positive regulation of axon extension involved in axon guidance; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell migration; retinal ganglion cell axon guidance; signal transduction; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway

NCBI Summary:This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:O14786
NCBI GenInfo Identifier:206729912
NCBI Gene ID:8829
NCBI Accession:O14786.3
UniProt Secondary Accession:O14786,O60461, Q5T7F1, Q5T7F2, Q5T7F3, Q86T59, Q96I90 Q96IH5, B0LPG9,
UniProt Related Accession:O14786
Molecular Weight:68,376 Da
NCBI Full Name:Neuropilin-1
NCBI Synonym Full Names:neuropilin 1
NCBI Official Symbol:NRP1  
NCBI Official Synonym Symbols:NP1; NRP; BDCA4; CD304; VEGF165R  
NCBI Protein Information:neuropilin-1
UniProt Protein Name:Neuropilin-1
UniProt Synonym Protein Names:Vascular endothelial cell growth factor 165 receptor; CD_antigen: CD304
Protein Family:Neuropilin
UniProt Gene Name:NRP1  
UniProt Entry Name:NRP1_HUMAN
Anti-NRP1 Antibody (CAB16697)Western blot analysis of extracts of various cell lines, using NRP1 antibody (CAB16697) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-NRP1 Antibody (CAB16697)Immunofluorescence analysis of C6 cells using NRP1 antibody (CAB16697) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-NRP1 Antibody (CAB16697)Immunofluorescence analysis of L929 cells using NRP1 antibody (CAB16697) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-NRP1 Antibody (CAB16697)Immunofluorescence analysis of U-2 OS cells using NRP1 antibody (CAB16697) at dilution of 1:100. Blue: DAPI for nuclear staining.
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