Immunology Antibodies 1
Anti-NRF2 Antibody (CAB1244)
- SKU:
- CAB1244
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-NRF2 Antibody |
| Antibody SKU: | CAB1244 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 366-605 of human NRF2 (NP_006155.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 366-605 of human NRF2 (NP_006155.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YGDT LLGL SDSE VEEL DSAP GSVK QNGP KTPV HSSG DMVQ PLSP SQGQ STHV HDAQ CENT PEKE LPVS PGHR KTPF TKDK HSSR LEAH LTRD ELRA KALH IPFP VEKI INLP VVDF NEMM SKEQ FNEA QLAL IRDI RRRG KNKV AAQN CRKR KLEN IVEL EQDL DHLK DEKE KLLK EKGE NDKS LHLL KKQL STLY LEVF SMLR DEDG KPYS PSEY SLQQ TRDG NVFL VPKS KKPD VKKN |
| Gene ID: | 4780 |
| Uniprot: | Q16236 |
| Cellular Location: | Cytoplasm, Nucleus, cytosol |
| Calculated MW: | 65kDa/66kDa/67kDa |
| Observed MW: | 100KDa |
| Synonyms: | NFE2L2, HEBP1, NRF2 |
| Background: | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. |
| UniProt Protein Function: | NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 2q31.2 Cellular Component: centrosome; cytoplasm; cytosol; nucleus; plasma membrane Molecular Function:DNA binding; protein binding; protein domain specific binding; transcription factor activity Biological Process: positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; transcription from RNA polymerase II promoter; unfolded protein response |
| NCBI Summary: | This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015] |
| UniProt Code: | Q16236 |
| NCBI GenInfo Identifier: | 25453452 |
| NCBI Gene ID: | 4780 |
| NCBI Accession: | Q16236.3 |
| UniProt Secondary Accession: | Q16236,Q53RW6, Q59HH2, Q96F71, B2RBU2, B4E338, E9PGJ7 |
| UniProt Related Accession: | Q16236 |
| Molecular Weight: | 65,356 Da |
| NCBI Full Name: | Nuclear factor erythroid 2-related factor 2 |
| NCBI Synonym Full Names: | nuclear factor, erythroid 2 like 2 |
| NCBI Official Symbol: | NFE2L2 |
| NCBI Official Synonym Symbols: | NRF2; HEBP1 |
| NCBI Protein Information: | nuclear factor erythroid 2-related factor 2 |
| UniProt Protein Name: | Nuclear factor erythroid 2-related factor 2 |
| UniProt Synonym Protein Names: | HEBP1; Nuclear factor, erythroid derived 2, like 2 |
| UniProt Gene Name: | NFE2L2 |
 | Western blot analysis of extracts of A-549 cells, using NRF2 Rabbit pAb (CAB1244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using NRF2 antibody (CAB1244) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of BALB-3T3 cells using NRF2 Rabbit pAb (CAB1244) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using NRF2 Rabbit pAb (CAB1244) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using NRF2 Rabbit pAb (CAB1244) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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