Epigenetics & Nuclear Signaling Antibodies 3
Anti-NRF1 Antibody (CAB5547)
(No reviews yet)
Write a Review
- SKU:
- CAB5547
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-NRF1 Antibody |
| Antibody SKU: | CAB5547 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-449 of human NRF1 (NP_001035199.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, MCF7, Mouse liver, Rat kidney, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-449 of human NRF1 (NP_001035199.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | WANV RSDV RTEE QKQR VSWT QALR TIVK NCYK QHGR EDLL YAFE DQQT QTQA TATH SIAH LVPS QTVV QTFS NPDG TVSL IQVG TGAT VATL ADAS ELPT TVTV AQVN YSAV ADGE VEQN WATL QGGE MTIQ TTQA SEAT QAVA SLAE AAVA ASQE MQQG ATVT MALN SEAA AHAV ATLA EATL QGGG QIVL SGET AAAV GALT GVQD AN |
| Gene ID: | 4899 |
| Uniprot: | Q16656 |
| Cellular Location: | Nucleus |
| Calculated MW: | 36kDa/46kDa/53kDa/55kDa |
| Observed MW: | 68kDa |
| Synonyms: | NRF1, ALPHA-PAL |
| Background: | This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for 'nuclear factor (erythroid-derived 2)-like 1' which has an official symbol of NFE2L1. |
| UniProt Protein Function: | NRF1: Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. Belongs to the NRF1/Ewg family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 7q32 Cellular Component: nucleoplasm; cytoplasm Biological Process: regulation of transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; generation of precursor metabolites and energy; organ regeneration; transcription, DNA-dependent; response to folic acid; organelle organization and biogenesis; response to hypoxia; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; response to electrical stimulus; response to estradiol stimulus |
| NCBI Summary: | This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014] |
| UniProt Code: | Q16656 |
| NCBI GenInfo Identifier: | 12643732 |
| NCBI Gene ID: | 4899 |
| NCBI Accession: | Q16656.1 |
| UniProt Secondary Accession: | Q16656,Q15305, Q96AN2, A8K4C6, B4DDV6, |
| UniProt Related Accession: | Q16656 |
| Molecular Weight: | 55,674 Da |
| NCBI Full Name: | Nuclear respiratory factor 1 |
| NCBI Synonym Full Names: | nuclear respiratory factor 1 |
| NCBI Official Symbol: | NRF1 |
| NCBI Official Synonym Symbols: | ALPHA-PAL |
| NCBI Protein Information: | nuclear respiratory factor 1; alpha palindromic-binding protein |
| UniProt Protein Name: | Nuclear respiratory factor 1 |
| UniProt Synonym Protein Names: | Alpha palindromic-binding protein; Alpha-pal |
| Protein Family: | Nuclear respiratory factor |
| UniProt Gene Name: | NRF1 |
| UniProt Entry Name: | NRF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NRF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
 | Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using NRF1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
View AllClose
Related Products
