Cell Biology Antibodies 3

Anti-NRAP Antibody (CAB12813)

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SKU:
CAB12813
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NRAP Antibody
Antibody SKU:CAB12813
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NRAP (NP_001248392.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:SH-SY5Y
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NRAP (NP_001248392.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNVQ PCSR CGYG VYPA EKIS CIDQ IWHK ACFH CEVC KMML SVNN FVSH QKKP YCHA HNPK NNTF TSVY HTPL NLNV RTFP EAIS GIHD QEDG EQCK SVFH WDMK SKDK EGAP NRQP LANE RAYW TGYG EGNA WCPG ALPD PEIV RMVE ARKS LGEE YTED YEQP RGKG SFPA MITP AYQR AKKA NQLA SQVE YKRG HDER ISRF STVV DTPE LLRS KAGA QLQS DVRY TEDY EQQR GKGS FPAM ITPA YQIA KRAN ELAS DVRY HQQY QKEM RGMA GPAI GAEG ILTR ECAD QYGQ GYPE
Gene ID:4892
Uniprot:Q86VF7
Cellular Location:
Calculated MW:192kDa/193kDa/197kDa
Observed MW:197kDa
Synonyms:NRAP, N-RAP
Background:
UniProt Protein Function:NRAP: May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: fascia adherens; muscle tendon junction

Molecular Function:actin binding; muscle alpha-actinin binding; protein binding

UniProt Code:Q86VF7
NCBI GenInfo Identifier:115502505
NCBI Gene ID:4892
NCBI Accession:Q86VF7.2
UniProt Secondary Accession:Q86VF7,O15500, Q5VWI3, Q5VWI4, Q6MZK3, Q6N026, Q6N059 Q6NSH8, Q6PDB0, Q719H6, Q86TC5, Q86TD6,
UniProt Related Accession:Q86VF7
Molecular Weight:197kDa
NCBI Full Name:Nebulin-related-anchoring protein
NCBI Synonym Full Names:nebulin related anchoring protein
NCBI Official Symbol:NRAP  
NCBI Official Synonym Symbols:N-RAP  
NCBI Protein Information:nebulin-related-anchoring protein
UniProt Protein Name:Nebulin-related-anchoring protein
Protein Family:Nebulin-related-anchoring protein
UniProt Gene Name:NRAP  
Anti-NRAP Antibody (CAB12813)Western blot analysis of extracts of SH-SY5Y cells, using NRAP antibody (CAB12813) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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