Antibody Name:	Anti-NR3C2 Antibody
Antibody SKU:	CAB3308
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human NR3C2 (NP_001159576.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, Mouse liver, Mouse kidney, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human NR3C2 (NP_001159576.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SWQR FYQL TKLL DSMH DLVS DLLE FCFY TFRE SHAL KVEF PAML VEII SDQL PKVE SGNA KPLY FHRK
Gene ID:	4306
Uniprot:	P08235
Cellular Location:	Cytoplasm, Endoplasmic reticulum membrane, Nucleus, Peripheral membrane protein
Calculated MW:	75kDa/94kDa/107kDa
Observed MW:	100KDa

Synonyms:

NR3C2, MCR, MLR, MR, NR3C2VIT

Background:

This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MCR: Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1). PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported. Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP). Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion. Belongs to the nuclear hormone receptor family. NR3 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor; DNA-binding Chromosomal Location of Human Ortholog: 4q31.1 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; receptor complex Molecular Function:protein binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; steroid binding Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression; steroid hormone mediated signaling; signal transduction Disease: Hypertension, Early-onset, Autosomal Dominant, With Severe Exacerbation In Pregnancy; Pseudohypoaldosteronism, Type I, Autosomal Dominant
NCBI Summary:	This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Code:	P08235
NCBI GenInfo Identifier:	126885
NCBI Gene ID:	4306
NCBI Accession:	P08235.1
UniProt Secondary Accession:	P08235,Q2NKL1, Q96KQ8, Q96KQ9, B0ZBF5, B0ZBF7,
UniProt Related Accession:	P08235
Molecular Weight:	984
NCBI Full Name:	Mineralocorticoid receptor
NCBI Synonym Full Names:	nuclear receptor subfamily 3, group C, member 2
NCBI Official Symbol:	NR3C2
NCBI Official Synonym Symbols:	MR; MCR; MLR; NR3C2VIT
NCBI Protein Information:	mineralocorticoid receptor; aldosterone receptor; mineralocorticoid receptor 1; mineralocorticoid receptor delta
UniProt Protein Name:	Mineralocorticoid receptor
UniProt Synonym Protein Names:	Nuclear receptor subfamily 3 group C member 2
Protein Family:	Malonyl-CoA reductase
UniProt Gene Name:	NR3C2
UniProt Entry Name:	MCR_HUMAN

	Western blot analysis of extracts of various cell lines, using NR3C2 antibody (CAB3308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00059). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using NR3C2 Rabbit pAb (CAB3308) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using NR3C2 Rabbit pAb (CAB3308) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using NR3C2 Rabbit pAb (CAB3308) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.