Antibody Name:	Anti-NR2C1 Antibody
Antibody SKU:	CAB6675
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NR2C1 (NP_003288.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HL-60, A-549, 22Rv1, SW480, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NR2C1 (NP_003288.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATI EEIA HQII EQQM GEIV TEQQ TGQK IQIV TALD HNTQ GKQF ILTN HDGS TPSK VILA RQDS TPGK VFLT TPDA AGVN QLFF TTPD LSAQ HLQL LTDN SPDQ GPNK VFDL CVVC GDKA SGRH YGAV TCEG CKGF FKRS IRKN LVYS CRGS KDCI INKH HRNR CQYC RLQR CIAF GMKQ DSVQ CERK PIEV SREK SSNC AAST EKIY IRKD LRSP LTAT PTFV TDSE STRS TGLL DSGM
Gene ID:	7181
Uniprot:	P13056
Cellular Location:	Nucleus, PML body
Calculated MW:	51kDa/53kDa/67kDa
Observed MW:	75kDa

Synonyms:

NR2C1, TR2

Background:

This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.

UniProt Protein Function:	NR2C1: Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid- regulated preadipocyte proliferation. Belongs to the nuclear hormone receptor family. NR2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Nuclear receptor Chromosomal Location of Human Ortholog: 12q22 Cellular Component: nucleoplasm; PML body Molecular Function:protein binding; protein homodimerization activity; DNA binding; zinc ion binding; sequence-specific DNA binding; histone deacetylase binding; steroid hormone receptor activity; receptor activity; transcription factor activity Biological Process: transcription initiation from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of retinoic acid receptor signaling pathway; gene expression; negative regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	P13056
NCBI GenInfo Identifier:	226693548
NCBI Gene ID:	7181
NCBI Accession:	P13056.2
UniProt Secondary Accession:	P13056,Q15625, Q15626, A8K5K4,
UniProt Related Accession:	P13056
Molecular Weight:	603
NCBI Full Name:	Nuclear receptor subfamily 2 group C member 1
NCBI Synonym Full Names:	nuclear receptor subfamily 2, group C, member 1
NCBI Official Symbol:	NR2C1
NCBI Official Synonym Symbols:	TR2
NCBI Protein Information:	nuclear receptor subfamily 2 group C member 1; orphan nuclear receptor TR2; TR2 nuclear hormone receptor; nuclear receptor subfamily 2, group C isoform
UniProt Protein Name:	Nuclear receptor subfamily 2 group C member 1
UniProt Synonym Protein Names:	Orphan nuclear receptor TR2; Testicular receptor 2
Protein Family:	Nuclear receptor subfamily
UniProt Gene Name:	NR2C1
UniProt Entry Name:	NR2C1_HUMAN

	Western blot analysis of extracts of various cell lines, using NR2C1 antibody (CAB6675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of MCF7 cells using NR2C1 antibody (CAB6675).