Immunology Antibodies 1
Anti-NR1H4 Antibody (CAB12788)
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- SKU:
- CAB12788
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-NR1H4 Antibody |
| Antibody SKU: | CAB12788 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 207-476 of human NR1H4 (NP_001193908.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 207-476 of human NR1H4 (NP_001193908.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CKSK RLRK NVKQ HADQ TVNE DSEG RDLR QVTS TTKS CREK TELT PDQQ TLLH FIMD SYNK QRMP QEIT NKIL KEEF SAEE NFLI LTEM ATNH VQVL VEFT KKLP GFQT LDHE DQIA LLKG SAVE AMFL RSAE IFNK KLPS GHSD LLEE RIRN SGIS DEYI TPMF SFYK SIGE LKMT QEEY ALLT AIVI LSPD RQYI KDRE AVEK LQEP LLDV LQKL CKIH QPEN PQHF ACLL GRLT ELRT FNHH HAEM LMSW RVND HKFT PLLC EIWD VQ |
| Gene ID: | 9971 |
| Uniprot: | Q96RI1 |
| Cellular Location: | Nucleus |
| Calculated MW: | 48kDa/54kDa/55kDa |
| Observed MW: | 56kDa |
| Synonyms: | NR1H4, BAR, FXR, HRR-1, HRR1, PFIC5, RIP14 |
| Background: | This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | NR1H4: Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. Heterodimer of NR1H4 and RXR. After activation by agonist binding, interacts with a coactivator, NCOA1 or NCOA2. Interacts with CARM1 and SMARD1. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor Chromosomal Location of Human Ortholog: 12q23.1 Cellular Component: nucleoplasm Molecular Function:bile acid binding; ligand-dependent nuclear receptor activity; ligand-dependent nuclear receptor binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity Biological Process: bile acid and bile salt transport; intracellular receptor-mediated signaling pathway; negative regulation of apoptosis; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; signal transduction; toll-like receptor 4 signaling pathway; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2016] |
| UniProt Code: | Q96RI1 |
| NCBI GenInfo Identifier: | 46577705 |
| NCBI Gene ID: | 9971 |
| NCBI Accession: | Q96RI1.2 |
| UniProt Secondary Accession: | Q96RI1,Q8NFP5, Q8NFP6, Q92943, A1L4K5, B7Z412, B7ZM06 F8VYG8, |
| UniProt Related Accession: | Q96RI1 |
| Molecular Weight: | 56kDa |
| NCBI Full Name: | Bile acid receptor |
| NCBI Synonym Full Names: | nuclear receptor subfamily 1 group H member 4 |
| NCBI Official Symbol: | NR1H4 |
| NCBI Official Synonym Symbols: | BAR; FXR; HRR1; HRR-1; PFIC5; RIP14 |
| NCBI Protein Information: | bile acid receptor |
| UniProt Protein Name: | Bile acid receptor |
| UniProt Synonym Protein Names: | Farnesoid X-activated receptor; Farnesol receptor HRR-1; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14 |
| Protein Family: | Bile acid receptor |
| UniProt Gene Name: | NR1H4 |
 | Western blot analysis of extracts of various cell lines, using NR1H4 antibody (CAB12788) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
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