Epigenetics & Nuclear Signaling Antibodies 3
Anti-NR0B1 Antibody (CAB1740)
- SKU:
- CAB1740
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-NR0B1 Antibody |
| Antibody SKU: | CAB1740 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human NR0B1 (NP_000466.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human NR0B1 (NP_000466.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGE NHQW QGSI LYNM LMSA KQTR AAPE APET RLVD QCWG CSCG DEPG VGRE GLLG GRNV ALLY RCCF CGKD HPRQ GSIL YSML TSAK QTYA APKA PEAT LGPC WGCS CGSD PGVG RAGL PGGR PVAL LYRC CFCG EDHP RQGS ILYS LLTS SKQT HVAP AAPE ARPG GAWW DRSY FAQR PGGK EALP GGRA TALL YRCC FCGE DHPQ QGST LYCV PTST NQAQ AAPE ERPR APWW DTSS GALR PVAL KSPQ VVCE AASA |
| Gene ID: | 190 |
| Uniprot: | P51843 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 43kDa/51kDa |
| Observed MW: | 50KDa |
| Synonyms: | NR0B1, AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2 |
| Background: | This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. |
| UniProt Protein Function: | NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:Nuclear receptor Chromosomal Location of Human Ortholog: Xp21.3 Cellular Component: nucleoplasm; polysomal ribosome; membrane; cytoplasm; nucleus Molecular Function:protein domain specific binding; DNA hairpin binding; protein binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; AF-2 domain binding; RNA binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription corepressor activity; transcription factor binding; steroid hormone receptor binding Biological Process: transcription initiation from RNA polymerase II promoter; hypothalamus development; negative regulation of steroid hormone receptor signaling pathway; intracellular receptor-mediated signaling pathway; gonad development; adrenal gland development; negative regulation of transcription factor activity; male gonad development; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell differentiation; protein localization; Leydig cell differentiation; pituitary gland development; male sex determination; steroid hormone mediated signaling; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; steroid biosynthetic process Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital |
| NCBI Summary: | This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P51843 |
| NCBI GenInfo Identifier: | 20532385 |
| NCBI Gene ID: | 190 |
| NCBI Accession: | P51843.2 |
| UniProt Related Accession: | P51843 |
| Molecular Weight: | |
| NCBI Full Name: | Nuclear receptor subfamily 0 group B member 1 |
| NCBI Synonym Full Names: | nuclear receptor subfamily 0 group B member 1 |
| NCBI Official Symbol: | NR0B1 |
| NCBI Official Synonym Symbols: | AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2 |
| NCBI Protein Information: | nuclear receptor subfamily 0 group B member 1 |
| UniProt Protein Name: | Nuclear receptor subfamily 0 group B member 1 |
| UniProt Synonym Protein Names: | DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1 |
| UniProt Gene Name: | NR0B1 |
| UniProt Entry Name: | NR0B1_HUMAN |
 | Western blot analysis of extracts of Mouse brain, using NR0B1 antibody (CAB1740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using NR0B1 antibody (CAB1740). Blue: DAPI for nuclear staining. |
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