Cell Biology Antibodies 9

Anti-NQO2 Antibody (CAB5440)

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SKU:
CAB5440
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NQO2 Antibody
Antibody SKU:CAB5440
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-231 of human NQO2 (NP_000895.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:20 - 1:50
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-231 of human NQO2 (NP_000895.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGK KVLI VYAH QEPK SFNG SLKN VAVD ELSR QGCT VTVS DLYA MNLE PRAT DKDI TGTL SNPE VFNY GVET HEAY KQRS LASD ITDE QKKV READ LVIF QFPL YWFS VPAI LKGW MDRV LCQG FAFD IPGF YDSG LLQG KLAL LSVT TGGT AEMY TKTG VNGD SRYF LWPL QHGT LHFC GFKV LAPQ ISFA PEIA SEEE RKGM VAAW SQRL QTIW KEEP IPCT AHWH FGQ
Gene ID:4835
Uniprot:P16083
Cellular Location:Cytoplasm
Calculated MW:25kDa
Observed MW:26kDa
Synonyms:NQO2, DHQV, DIA6, NMOR2, QR2
Background:This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:NQO2: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Belongs to the NAD(P)H dehydrogenase (quinone) family.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.10.99.2

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: nucleoplasm; cytoplasm

Molecular Function:electron carrier activity; dihydronicotinamide riboside quinone reductase activity; NADPH dehydrogenase (quinone) activity; metal ion binding

Biological Process: memory

Disease: Breast Cancer

NCBI Summary:This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
UniProt Code:P16083
NCBI GenInfo Identifier:317373581
NCBI Gene ID:4835
NCBI Accession:P16083.5
UniProt Secondary Accession:P16083,Q9JI75,
UniProt Related Accession:P16083
Molecular Weight:231
NCBI Full Name:Ribosyldihydronicotinamide dehydrogenase
NCBI Synonym Full Names:NAD(P)H dehydrogenase, quinone 2
NCBI Official Symbol:NQO2  
NCBI Official Synonym Symbols:QR2; DHQV; DIA6; NMOR2  
NCBI Protein Information:ribosyldihydronicotinamide dehydrogenase [quinone]; ribosyldihydronicotinamide dehydrogenase [quinone]; quinone reductase 2; NRH:quinone oxidoreductase 2; NAD(P)H menadione oxidoreductase-1, dioxin-inducible-2
UniProt Protein Name:Ribosyldihydronicotinamide dehydrogenase [quinone]
UniProt Synonym Protein Names:NRH dehydrogenase [quinone] 2; NRH:quinone oxidoreductase 2; Quinone reductase 2; QR2
Protein Family:NADH-quinone oxidoreductase
UniProt Gene Name:NQO2  
UniProt Entry Name:NQO2_HUMAN
Anti-NQO2 Antibody (CAB5440)Western blot analysis of extracts of HeLa cells, using NQO2 antibody (CAB5440) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
Anti-NQO2 Antibody (CAB5440)Immunofluorescence analysis of U2OS cells using NQO2 antibody (CAB5440). Blue: DAPI for nuclear staining.
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