Signal Transduction Antibodies 2

Anti-NPLOC4 Antibody (CAB3256)

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SKU:
CAB3256
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-NPLOC4 Antibody
Antibody SKU:CAB3256
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NPLOC4 (NP_060391.2).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-549, LO2, 293T, Mouse liver, Mouse brain, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NPLOC4 (NP_060391.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAES IIIR VQSP DGVK RITA TKRE TAAT FLKK VAKE FGFQ NNGF SVYI NRNK TGEI TASS NKSL NLLK IKHG DLLF LFPS SLAG PSSE METS VPPG FKVF GAPN VVED EIDQ YLSK QDGK IYRS RDPQ LCRH GPLG KCVH CVPL EPFD EDYL NHLE PPVK HMSF HAYI RKLT GGAD KGKF VALE NISC KIKS GCEG HLPW PNGI CTKC QPSA ITLN RQKY RHVD NIMF ENHT VADR FLDF WRKT GNQH FGYL YGRY TEHK DIPL GIRA EV
Gene ID:55666
Uniprot:Q8TAT6
Cellular Location:Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol
Calculated MW:68kDa/69kDa
Observed MW:70kDa
Synonyms:NPLOC4, NPL4, NPL4 homolog
Background:
UniProt Protein Function:NPL4: The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Belongs to the NPL4 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 17qter

Cellular Component: endoplasmic reticulum; nuclear envelope-endoplasmic reticulum network; nucleoplasm; nucleus

Molecular Function:protein binding; ubiquitin binding; ubiquitin protein ligase binding

Biological Process: ER-associated protein catabolic process; Golgi organization and biogenesis; retrograde protein transport, ER to cytosol; ubiquitin-dependent protein catabolic process

UniProt Code:Q8TAT6
NCBI GenInfo Identifier:50428974
NCBI Gene ID:55666
NCBI Accession:Q8TAT6.3
UniProt Secondary Accession:Q8TAT6,Q8N3J1, Q9H8V2, Q9H964, Q9NWR5, Q9P229,
UniProt Related Accession:Q8TAT6
Molecular Weight:69,461 Da
NCBI Full Name:Nuclear protein localization protein 4 homolog
NCBI Synonym Full Names:NPL4 homolog, ubiquitin recognition factor
NCBI Official Symbol:NPLOC4  
NCBI Official Synonym Symbols:NPL4  
NCBI Protein Information:nuclear protein localization protein 4 homolog
UniProt Protein Name:Nuclear protein localization protein 4 homolog
Protein Family:Nuclear protein localization protein
UniProt Gene Name:NPLOC4  
UniProt Entry Name:NPL4_HUMAN
Anti-NPLOC4 Antibody (CAB3256)Western blot analysis of extracts of various cell lines, using NPLOC4 antibody (CAB3256) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-NPLOC4 Antibody (CAB3256)Immunofluorescence analysis of L929 cells using NPLOC4 Rabbit pAb (CAB3256) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-NPLOC4 Antibody (CAB3256)Immunofluorescence analysis of U2OS cells using NPLOC4 Rabbit pAb (CAB3256) at dilution of 1:100. Blue: DAPI for nuclear staining.
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