Cell Biology Antibodies 7

Anti-NPHS2 Antibody (CAB17337)

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SKU:
CAB17337
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NPHS2 Antibody
Antibody SKU:CAB17337
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human NPHS2.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, 293T, mouse testis, mouse kidney, mouse brain, rat spleen
Immunogen:Recombinant protein of human NPHS2.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:7827
Uniprot:Q9NP85
Cellular Location:
Calculated MW:42kDa
Observed MW:42kDa
Synonyms:NPHS2, PDCN, SRN1, podocin
Background:This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: protein complex; integral to plasma membrane; endoplasmic reticulum; plasma membrane; intercellular junction; lipid raft

Molecular Function:protein binding

Biological Process: actin cytoskeleton reorganization; excretion

Disease: Nephrotic Syndrome, Type 2

NCBI Summary:This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:Q9NP85
NCBI GenInfo Identifier:12230467
NCBI Gene ID:7827
NCBI Accession:Q9NP85.1
UniProt Secondary Accession:Q9NP85,Q8N6Q5, B1AM32, B1AM33,
UniProt Related Accession:Q9NP85
Molecular Weight:34,421 Da
NCBI Full Name:Podocin
NCBI Synonym Full Names:nephrosis 2, idiopathic, steroid-resistant (podocin)
NCBI Official Symbol:NPHS2  
NCBI Official Synonym Symbols:PDCN; SRN1  
NCBI Protein Information:podocin
UniProt Protein Name:Podocin
Protein Family:Podocin
UniProt Gene Name:NPHS2  
UniProt Entry Name:PODO_HUMAN
Anti-NPHS2 Antibody (CAB17337)Western blot analysis of extracts of various cell lines, using NPHS2 antibody (CAB17337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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