Metabolism Antibodies 1

Anti-NPC1L1 Antibody (CAB10049)

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SKU:
CAB10049
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-NPC1L1 Antibody
Antibody SKU:CAB10049
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1030-1100 of human NPC1L1 (NP_001095118.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:4000
Reactivity:Human
Positive Samples:HepG2, 293T, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1030-1100 of human NPC1L1 (NP_001095118.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AAYS TSVN LTSD GQVL ASRF MAYH KPLK NSQD YTEA LRAA RELA ANIT ADLR KVPG TDPA FEVF PYTI TNV
Gene ID:29881
Uniprot:Q9UHC9
Cellular Location:Apical cell membrane, Cell membrane, Cytoplasmic vesicle membrane, Multi-pass membrane protein
Calculated MW:79kDa/140kDa/145kDa/148kDa
Observed MW:148kDa
Synonyms:NPC1L1, NPC11L1
Background:The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:NPC1L1: Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Interacts with RAB11A, MYO5B and RAB11FIP2. Interaction with RAB11A, MYO5B and RAB11FIP2 is required for proper transport to the plasma membrane upon cholesterol depletion. Expression is decreased in Caco-2 cells upon PPARD activation. Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. Belongs to the patched family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: apical plasma membrane; brush border membrane; cytoplasmic vesicle membrane; integral component of membrane; plasma membrane

Molecular Function:drug binding; myosin V binding; protein binding; Rab GTPase binding

Biological Process: cholesterol biosynthetic process; cholesterol transport; intestinal cholesterol absorption; lipoprotein metabolic process; response to drug

NCBI Summary:The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:Q9UHC9
NCBI GenInfo Identifier:156231353
NCBI Gene ID:29881
NCBI Accession:NP_001095118.1
UniProt Related Accession:Q9UHC9
Molecular Weight:149kDa
NCBI Full Name:NPC1-like intracellular cholesterol transporter 1 isoform 2
NCBI Synonym Full Names:NPC1 like intracellular cholesterol transporter 1
NCBI Official Symbol:NPC1L1  
NCBI Official Synonym Symbols:LDLCQ7; NPC11L1; SLC65A2  
NCBI Protein Information:NPC1-like intracellular cholesterol transporter 1
UniProt Protein Name:Niemann-Pick C1-like protein 1
Protein Family:Niemann-Pick C1-like protein
UniProt Gene Name:NPC1L1  
Anti-NPC1L1 Antibody (CAB10049)Western blot analysis of extracts of various cell lines, using NPC1L1 antibody (CAB10049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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