Cell Biology Antibodies 7
Anti-NOX2 / gp91phox Antibody (CAB1636)
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- SKU:
- CAB1636
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NOX2 / gp91phox Antibody |
| Antibody SKU: | CAB1636 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 283-570 of human NOX2 / gp91phox (NP_000388.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, LO2, HL-60, THP-1, BT-474, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 283-570 of human NOX2 / gp91phox (NP_000388.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ERLV RFWR SQQK VVIT KVVT HPFK TIEL QMKK KGFK MEVG QYIF VKCP KVSK LEWH PFTL TSAP EEDF FSIH IRIV GDWT EGLF NACG CDKQ EFQD AWKL PKIA VDGP FGTA SEDV FSYE VVML VGAG IGVT PFAS ILKS VWYK YCNN ATNL KLKK IYFY WLCR DTHA FEWF ADLL QLLE SQMQ ERNN AGFL SYNI YLTG WDES QANH FAVH HDEE KDVI TGLK QKTL YGRP NWDN EFKT IASQ HPNT RIGV FLCG PEAL AETL SKQS ISNS ESGP RGVH FIFN KENF |
| Gene ID: | 1536 |
| Uniprot: | P04839 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 65kDa |
| Observed MW: | 65kDa |
| Synonyms: | AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX, CYBB |
| Background: | Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. |
| UniProt Protein Function: | CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; EC 1.-.-.-; Membrane protein, integral; Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: Xp21.1 Cellular Component: Golgi apparatus; phagocytic vesicle membrane; cell soma; mitochondrion; rough endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; NADPH oxidase complex Molecular Function:protein binding; FAD binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; voltage-gated ion channel activity Biological Process: response to drug; respiratory burst; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; ion transport; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; response to nutrient; hydrogen peroxide biosynthetic process Disease: Immunodeficiency 34; Granulomatous Disease, Chronic, X-linked |
| NCBI Summary: | Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P04839 |
| NCBI GenInfo Identifier: | 115211 |
| NCBI Gene ID: | 1536 |
| NCBI Accession: | P04839.2 |
| UniProt Secondary Accession: | P04839,Q2PP16, A8K138, |
| UniProt Related Accession: | P04839 |
| Molecular Weight: | 65,336 Da |
| NCBI Full Name: | Cytochrome b-245 heavy chain |
| NCBI Synonym Full Names: | cytochrome b-245, beta polypeptide |
| NCBI Official Symbol: | CYBB |
| NCBI Official Synonym Symbols: | CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX |
| NCBI Protein Information: | cytochrome b-245 heavy chain; CGD91-phox; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox; superoxide-generating NADPH oxidase heavy chain subunit |
| UniProt Protein Name: | Cytochrome b-245 heavy chain |
| UniProt Synonym Protein Names: | CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYBB |
| UniProt Entry Name: | CY24B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NOX2 / gp91phox antibody (CAB1636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat bone marrow using NOX2/gp91phox antibody (CAB1636) at dilution of 1:200 (40x lens). |
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