Metabolism Antibodies 2
Anti-NOTUM Antibody (CAB16600)
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- SKU:
- CAB16600
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-NOTUM Antibody |
| Antibody SKU: | CAB16600 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human NOTUM (NP_848588.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | BxPC-3, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human NOTUM (NP_848588.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SEGR KTWR RRGQ QPPP PPRT EAAP AAGQ PVES FPLD FTAV EGNM DSFM AQVK SLAQ SLYP CSAQ QLNE DLRL HLLL NTSV TCND GSPA GYYL KESR GSRR WLLF LEGG WYCF NREN CDSR YDTM RRLM SSRD WPRT RTGT GILS SQPE ENPY WWNA NMVF IPYC SSDV WSGA SSKS EKNE YAFM GALI IQEV VREL LGRG LSGA KVLL LAGS SAGG TGVL LNVD RVAE QLEK LGYP AIQV RGLA DSGW FLDN KQYR HTDC VDTI TCAP TEAI RRGI RYWN GVVP ERCR RQFQ EGEE WNCF F |
| Gene ID: | 147111 |
| Uniprot: | Q6P988 |
| Cellular Location: | Secreted |
| Calculated MW: | 55kDa |
| Observed MW: | 56kDa |
| Synonyms: | NOTUM, hNOTUM |
| Background: |
| UniProt Protein Function: | NOTUM: May deacetylate GlcNAc residues on cell surface glycans (Potential). Belongs to the pectinacetylesterase family. |
| UniProt Protein Details: | Protein type:EC 3.-.-.-; Secreted; Hydrolase; Secreted, signal peptide Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: extracellular region Molecular Function:phospholipase C activity Biological Process: negative regulation of Wnt receptor signaling pathway |
| UniProt Code: | Q6P988 |
| NCBI GenInfo Identifier: | 182628300 |
| NCBI Gene ID: | 147111 |
| NCBI Accession: | Q6P988.2 |
| UniProt Secondary Accession: | Q6P988,Q8N410, Q8NI82, |
| UniProt Related Accession: | Q6P988 |
| Molecular Weight: | 55,699 Da |
| NCBI Full Name: | Palmitoleoyl-protein carboxylesterase NOTUM |
| NCBI Synonym Full Names: | NOTUM, palmitoleoyl-protein carboxylesterase |
| NCBI Official Symbol: | NOTUM |
| NCBI Official Synonym Symbols: | hNOTUM |
| NCBI Protein Information: | palmitoleoyl-protein carboxylesterase NOTUM |
| UniProt Protein Name: | Palmitoleoyl-protein carboxylesterase NOTUM |
| UniProt Synonym Protein Names: | hNOTUM |
| Protein Family: | Palmitoleoyl-protein carboxylesterase |
| UniProt Gene Name: | NOTUM |
| UniProt Entry Name: | NOTUM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NOTUM antibody (CAB16600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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