Signal Transduction Antibodies 1

Anti-NOSIP Antibody (CAB10024)

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SKU:
CAB10024
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-NOSIP Antibody
Antibody SKU:CAB10024
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human NOSIP (NP_057037.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549, B cells, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human NOSIP (NP_057037.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTRH GKNC TAGA VYTY HEKK KDTA ASGY GTQN IRLS RDAV KDFD CCCL SLQP CHDP VVTP DGYL YERE AILE YILH QKKE IARQ MKAY EKQR GTRR EEQK ELQR AASQ DHVR GFLE KESA IVSR PLNP FTAK ALSG TSPD DVQP GPSV GPPS KDKD KVLP SFWI PSLT PEAK ATKL EKPS RTVT CPMS GKPL RMSD LTPV HFTP LDSS VDRV GLIT RSER YVCA VTRD SLSN ATPC AVLR PSGA VVTL ECVE KLIR KDMV DPVT GDKL TDRD IIVL QRGG TGFA GSGV KLQA EKSR PVMQ A
Gene ID:51070
Uniprot:Q9Y314
Cellular Location:Cytoplasm, Nucleus
Calculated MW:33kDa
Observed MW:35kDa
Synonyms:NOSIP, CGI-25
Background:The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein.
UniProt Protein Function:NOSIP: Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity. Belongs to the NOSIP family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytoplasm; cytosol; Golgi membrane; nucleus

Molecular Function:protein binding

Biological Process: negative regulation of catalytic activity; negative regulation of nitric-oxide synthase activity; regulation of nitric-oxide synthase activity

NCBI Summary:The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012]
UniProt Code:Q9Y314
NCBI GenInfo Identifier:74735248
NCBI Gene ID:51070
NCBI Accession:Q9Y314.1
UniProt Secondary Accession:Q9Y314,Q96FD2,
UniProt Related Accession:Q9Y314
Molecular Weight:33,172 Da
NCBI Full Name:Nitric oxide synthase-interacting protein
NCBI Synonym Full Names:nitric oxide synthase interacting protein
NCBI Official Symbol:NOSIP  
NCBI Official Synonym Symbols:CGI-25  
NCBI Protein Information:nitric oxide synthase-interacting protein
UniProt Protein Name:Nitric oxide synthase-interacting protein
UniProt Synonym Protein Names:E3 ubiquitin-protein ligase NOSIP (EC:6.3.2.-); eNOS-interacting protein
Protein Family:Nitric oxide synthase-interacting protein
UniProt Gene Name:NOSIP  
UniProt Entry Name:NOSIP_HUMAN
Anti-NOSIP Antibody (CAB10024)Western blot analysis of extracts of various cell lines, using NOSIP antibody (CAB10024) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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