Cell Biology Antibodies 4

Anti-NOS2 Antibody (CAB14031)

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SKU:
CAB14031
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NOS2 Antibody
Antibody SKU:CAB14031
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-730 of human NOS2 (NP_000616.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:RAW264.7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-730 of human NOS2 (NP_000616.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VWQD EKRR PKRR EIPL KVLV KAVL FACM LMRK TMAS RVRV TILF ATET GKSE ALAW DLGA LFSC AFNP KVVC MDKY RLSC LEEE RLLL VVTS TFGN GDCP GNGE KLKK SLFM LKEL NNKF RYAV FGLG SSMY PRFC AFAH DIDQ KLSH LGAS QLTP MGEG DELS GQED AFRS WAVQ TFKA ACET FDVR GKQH IQIP KLYT SNVT WDPH HYRL VQDS QPLD LSKA LSSM HAK
Gene ID:4843
Uniprot:P35228
Cellular Location:
Calculated MW:126kDa/131kDa
Observed MW:131KDa
Synonyms:NOS, INOS, NOS2A, HEP-NOS, iNOS, NOS2
Background:Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
UniProt Protein Function:Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
UniProt Protein Details:

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1.

Tissue specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Induction: By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Ref.14

Polymorphism: Note=Genetic variations in NOS2 are involved in resistance to malaria [

MIM:611162].

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.

NCBI Summary:Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
UniProt Code:P35228
NCBI GenInfo Identifier:1352513
NCBI Gene ID:4843
NCBI Accession:P35228.2
UniProt Secondary Accession:P35228,O60757, O94994, Q16263, Q16692, Q4TTS5, Q9UD42 A1L3U5, B7ZLY2,
UniProt Related Accession:P35228
Molecular Weight:131,117 Da
NCBI Full Name:Nitric oxide synthase, inducible
NCBI Synonym Full Names:nitric oxide synthase 2, inducible
NCBI Official Symbol:NOS2  
NCBI Official Synonym Symbols:NOS; INOS; NOS2A; HEP-NOS  
NCBI Protein Information:nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
UniProt Protein Name:Nitric oxide synthase, inducible
UniProt Synonym Protein Names:Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Protein Family:Inositol 3-kinase
UniProt Gene Name:NOS2  
UniProt Entry Name:NOS2_HUMAN
Anti-NOS2 Antibody (CAB14031)Western blot analysis of extracts of RAW264.7 cells, using NOS2 antibody (CAB14031) at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 ug/ml) at 37'C for over night. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-NOS2 Antibody (CAB14031)Immunohistochemistry of paraffin-embedded mouse kidney using NOS2 antibody (CAB14031) at dilution of 1:100 (40x lens).
Anti-NOS2 Antibody (CAB14031)Immunofluorescence analysis of U2OS cells using NOS2 antibody (CAB14031) at dilution of 1:100. Blue: DAPI for nuclear staining.
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