Cell Biology Antibodies 9
Anti-NOLC1 Antibody (CAB5899)
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- SKU:
- CAB5899
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NOLC1 Antibody |
| Antibody SKU: | CAB5899 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-699 of human NOLC1 (NP_004732.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HeLa, HepG2, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-699 of human NOLC1 (NP_004732.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VKPA AAPK QPVG GGQK LLTR KADS SSSE EESS SSEE EKTK KMVA TTKP KATA KAAL SLPA KQAP QGSR DSSS DSDS SSSE EEEE KTSK SAVK KKPQ KVAG GAAP SKPA SAKK GKAE SSNS SSSD DSSE EEEE KLKG KGSP RPQA PKAN GTSA LTAQ NGKA AKNS EEEE EEKK KAAV VVSK SGSL KKRK QNEA AKEA ETPQ AKKI KLQT PNTF PKRK KGEK RASS PFRR VREE EIEV DSRV ADNS FDAK RGAA GDWG ERAN QVLK FTKG KSFR HEKT KKKR GSYR GGSI SVQV NSIK FDSE |
| Gene ID: | 9221 |
| Uniprot: | Q14978 |
| Cellular Location: | Cytoplasm, Nucleus, nucleolus |
| Calculated MW: | 73kDa/74kDa |
| Observed MW: | 120kDa |
| Synonyms: | NOLC1, NOPP130, NOPP140, NS5ATP13, P130 |
| Background: |
| UniProt Protein Function: | NOLC1: Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA-binding; Transcription, coactivator/corepressor; Nucleolus Chromosomal Location of Human Ortholog: 10q24.32 Cellular Component: nucleolus; nucleoplasm Molecular Function:protein binding Biological Process: cell cycle; mitosis; rRNA processing |
| UniProt Code: | Q14978 |
| NCBI GenInfo Identifier: | 145559503 |
| NCBI Gene ID: | 9221 |
| NCBI Accession: | Q14978.2 |
| UniProt Secondary Accession: | Q14978,Q15030, Q5VV70, Q9BUV3, |
| UniProt Related Accession: | Q14978 |
| Molecular Weight: | 73,745 Da |
| NCBI Full Name: | Nucleolar and coiled-body phosphoprotein 1 |
| NCBI Synonym Full Names: | nucleolar and coiled-body phosphoprotein 1 |
| NCBI Official Symbol: | NOLC1 |
| NCBI Official Synonym Symbols: | P130; NOPP130; NOPP140; NS5ATP13 |
| NCBI Protein Information: | nucleolar and coiled-body phosphoprotein 1 |
| UniProt Protein Name: | Nucleolar and coiled-body phosphoprotein 1 |
| UniProt Synonym Protein Names: | 140 kDa nucleolar phosphoprotein; Nopp140; Hepatitis C virus NS5A-transactivated protein 13; HCV NS5A-transactivated protein 13; Nucleolar 130 kDa protein; Nucleolar phosphoprotein p130 |
| Protein Family: | Nucleolar and coiled-body phosphoprotein |
| UniProt Gene Name: | NOLC1 |
| UniProt Entry Name: | NOLC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NOLC1 antibody (CAB5899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U-2 OS cells using NOLC1 antibody (CAB5899) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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