Name: Anti-NOL6 Antibody (CAB14420)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB14420
Availability: OutOfStock

Antibody Name:	Anti-NOL6 Antibody
Antibody SKU:	CAB14420
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1032-1146 of human NOL6 (NP_075068.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, A-431, HeLa, SGC-7901, Mouse lung, Mouse brain, Mouse liver, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1032-1146 of human NOL6 (NP_075068.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SFCR GLLS QPGP SSLM PVLG YDPP QLYL TQLR EAFG DLAL FFYD QHGG EVIG VLWK PTSF QPQP FKAS STKG RMVM SRGG ELVM VPNV EAIL EDFA VLGE GLVQ TVEA RSER WTV
Gene ID:	65083
Uniprot:	Q9H6R4
Cellular Location:	Chromosome, Nucleus, nucleolus
Calculated MW:	77kDa/112kDa/127kDa
Observed MW:	127kDa

Synonyms:

NOL6, NRAP, UTP22, bA311H10.1

Background:

The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified.

UniProt Protein Function:	NOL6: The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Nucleolus Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: condensed nuclear chromosome; mitochondrion; nucleolus; nucleus; small subunit processome Molecular Function:protein binding Biological Process: rRNA processing; tRNA export from nucleus
NCBI Summary:	The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H6R4
NCBI GenInfo Identifier:	18644728
NCBI Gene ID:	65083
NCBI Accession:	NP_075068.2
UniProt Secondary Accession:	Q9H6R4,Q5T5M3, Q5T5M4, Q7L4G6, Q8N6I0, Q8TEY9, Q8TEZ0 Q8TEZ1, Q9H675,
UniProt Related Accession:	Q9H6R4
Molecular Weight:	127,320 Da
NCBI Full Name:	nucleolar protein 6 alpha isoform
NCBI Synonym Full Names:	nucleolar protein 6
NCBI Official Symbol:	NOL6
NCBI Official Synonym Symbols:	NRAP; UTP22; bA311H10.1
NCBI Protein Information:	nucleolar protein 6
UniProt Protein Name:	Nucleolar protein 6
UniProt Synonym Protein Names:	Nucleolar RNA-associated protein; Nrap
Protein Family:	Nucleolar protein
UniProt Gene Name:	NOL6
UniProt Entry Name:	NOL6_HUMAN

Western blot analysis of extracts of various cell lines, using NOL6 antibody (CAB14420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

NOL6 Antibody (PACO10889)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)