Cell Biology Antibodies 10

Anti-NNMT Antibody (CAB6673)

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SKU:
CAB6673
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NNMT Antibody
Antibody SKU:CAB6673
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human NNMT (NP_006160.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, SKOV3, M21, Mouse liver, Mouse brain, Rat lung, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human NNMT (NP_006160.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MESG FTSK DTYL SHFN PRDY LEKY YKFG SRHS AESQ ILKH LLKN LFKI FCLD GVKG DLLI DIGS GPTI YQLL SACE SFKE IVVT DYSD QNLQ ELEK WLKK EPEA FDWS PVVT YVCD LEGN RVKG PEKE EKLR QAVK QVLK CDVT QSQP LGAV PLPP ADCV LSTL CLDA ACPD LPTY CRAL RNLG SLLK PGGF LVIM DALK SSYY MIGE QKFS SLPL GREA VEAA VKEA GYTI EWFE VISQ SYSS TMAN NEGL FSLV ARKL SRPL
Gene ID:4837
Uniprot:P40261
Cellular Location:Cytoplasm
Calculated MW:29kDa
Observed MW:30kDa
Synonyms:NNMT
Background:N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
UniProt Protein Function:NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.
UniProt Protein Details:

Protein type:Methyltransferase; EC 2.1.1.1; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function:nicotinamide N-methyltransferase activity

Biological Process: response to drug; methylation; organ regeneration; xenobiotic metabolic process; response to organic nitrogen

NCBI Summary:N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]
UniProt Code:P40261
NCBI GenInfo Identifier:730163
NCBI Gene ID:4837
NCBI Accession:P40261.1
UniProt Related Accession:P40261
Molecular Weight:
NCBI Full Name:Nicotinamide N-methyltransferase
NCBI Synonym Full Names:nicotinamide N-methyltransferase
NCBI Official Symbol:NNMT  
NCBI Protein Information:nicotinamide N-methyltransferase
UniProt Protein Name:Nicotinamide N-methyltransferase
UniProt Gene Name:NNMT  
UniProt Entry Name:NNMT_HUMAN
Anti-NNMT Antibody (CAB6673)Western blot analysis of extracts of various cell lines, using NNMT antibody (CAB6673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NNMT Antibody (CAB6673)Immunofluorescence analysis of human liver cancer cells using NNMT antibody (CAB6673) at dilution of 1:100. Blue: DAPI for nuclear staining.
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