Cell Biology Antibodies 12
Anti-NMT1 Antibody (CAB8724)
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- SKU:
- CAB8724
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NMT1 Antibody |
| Antibody SKU: | CAB8724 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NMT1 (NP_066565.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, HL-60, SW480, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NMT1 (NP_066565.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADE SETA VKPP APPL PQMM EGNG NGHE HCSD CENE EDNS YNRG GLSP ANDT GAKK KKKK QKKK KEKG SETD SAQD QPVK MNSL PAER IQEI QKAI ELFS VGQG PAKT MEEA SKRS YQFW DTQP VPKL GEVV NTHG PVEP DKDN IRQE PYTL PQGF TWDA LDLG DRGV LKEL YTLL NENY VEDD DNMF RFDY SPEF LLWA LRPP GWLP QWHC GVRV VSSR KLVG FISA IPAN IHIY DTEK KMVE INFL CV |
| Gene ID: | 4836 |
| Uniprot: | P30419 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein, cytosol |
| Calculated MW: | 48kDa/56kDa |
| Observed MW: | 57kDa |
| Synonyms: | NMT1, NMT |
| Background: |
| UniProt Protein Function: | NMT1: Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Belongs to the NMT family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.3.1.97; Motility/polarity/chemotaxis; Transferase Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: cytoplasm; cytosol; extrinsic component of membrane; mitochondrion; plasma membrane Molecular Function:glycylpeptide N-tetradecanoyltransferase activity; myristoyltransferase activity Biological Process: in utero embryonic development; ketone metabolic process; N-terminal peptidyl-glycine N-myristoylation; N-terminal protein myristoylation; positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; regulation of rhodopsin mediated signaling |
| NCBI Summary: | Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008] |
| UniProt Code: | P30419 |
| NCBI GenInfo Identifier: | 10835073 |
| NCBI Gene ID: | 4836 |
| NCBI Accession: | NP_066565.1 |
| UniProt Secondary Accession: | P30419,Q9UE09, A8K7C1, |
| UniProt Related Accession: | P30419 |
| Molecular Weight: | 48,141 Da |
| NCBI Full Name: | glycylpeptide N-tetradecanoyltransferase 1 |
| NCBI Synonym Full Names: | N-myristoyltransferase 1 |
| NCBI Official Symbol: | NMT1 |
| NCBI Official Synonym Symbols: | NMT |
| NCBI Protein Information: | glycylpeptide N-tetradecanoyltransferase 1 |
| UniProt Protein Name: | Glycylpeptide N-tetradecanoyltransferase 1 |
| UniProt Synonym Protein Names: | Myristoyl-CoA:protein N-myristoyltransferase 1; NMT 1; Type I N-myristoyltransferase; Peptide N-myristoyltransferase 1 |
| Protein Family: | Glycylpeptide N-tetradecanoyltransferase |
| UniProt Gene Name: | NMT1 |
 | Western blot analysis of extracts of various cell lines, using NMT1 antibody (CAB8724) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using NMT1 antibody (CAB8724) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using NMT1 antibody (CAB8724) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human mammary cancer using NMT1 antibody (CAB8724) at dilution of 1:100 (40x lens). |
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