Antibody Name:	Anti-NMNAT3 Antibody
Antibody SKU:	CAB14465
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human NMNAT3 (NP_835471.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse kidney, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human NMNAT3 (NP_835471.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MYQV IQGI ISPV NDTY GKKD LAAS HHRV AMAR LALQ TSDW IRVD PWES EQAQ WMET VKVL RHHH SKLL RSPP QMEG PDHG KALF STPA AVPE LKLL CGAD VLKT FQTP NLWK DAHI QEIV EKFG LVCV GRVG HDPK GYIA ESPI LRMH QHNI HLAK EPVQ NEIS ATYI RRAL GQGQ SVKY LIPD AVIT YIKD HGLY TKGS TWKG KSTQ STEG KTS
Gene ID:	349565
Uniprot:	Q96T66
Cellular Location:	Mitochondrion
Calculated MW:	18kDa/24kDa/28kDa
Observed MW:	28kDa

Synonyms:

NMNAT3, FKSG76, PNAT-3, PNAT3

Background:

This gene encodes a member of the nicotinamide/nicotinic acid mononucleotide adenylyltransferase family. These enzymes use ATP to catalyze the synthesis of nicotinamide adenine dinucleotide or nicotinic acid adenine dinucleotide from nicotinamide mononucleotide or nicotinic acid mononucleotide, respectively. The encoded protein is localized to mitochondria and may also play a neuroprotective role as a molecular chaperone. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	NMNAT3: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD (+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following injury. Belongs to the eukaryotic NMN adenylyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.7.7.1; EC 2.7.7.18; Apoptosis; Mitochondrial; Transferase Chromosomal Location of Human Ortholog: 3q23 Cellular Component: cytosol; mitochondrion Molecular Function:nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity Biological Process: NAD biosynthetic process; NAD metabolic process
NCBI Summary:	This gene encodes a member of the nicotinamide/nicotinic acid mononucleotide adenylyltransferase family. These enzymes use ATP to catalyze the synthesis of nicotinamide adenine dinucleotide or nicotinic acid adenine dinucleotide from nicotinamide mononucleotide or nicotinic acid mononucleotide, respectively. The encoded protein is localized to mitochondria and may also play a neuroprotective role as a molecular chaperone. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:	Q96T66
NCBI GenInfo Identifier:	116242680
NCBI Gene ID:	349565
NCBI Accession:	Q96T66.2
UniProt Secondary Accession:	Q96T66,Q8N4G1, B3KVR6, D3DNF2, D3DNF3,
UniProt Related Accession:	Q96T66
Molecular Weight:	18,255 Da
NCBI Full Name:	Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3
NCBI Synonym Full Names:	nicotinamide nucleotide adenylyltransferase 3
NCBI Official Symbol:	NMNAT3
NCBI Official Synonym Symbols:	PNAT3; FKSG76; PNAT-3
NCBI Protein Information:	nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3
UniProt Protein Name:	Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3
UniProt Synonym Protein Names:	Nicotinamide-nucleotide adenylyltransferase 3; NMN adenylyltransferase 3; Nicotinate-nucleotide adenylyltransferase 3 (EC:2.7.7.18); NaMN adenylyltransferase 3; Pyridine nucleotide adenylyltransferase 3 (EC:2.7.7.1); PNAT-3
Protein Family:	Nicotinamide/nicotinic acid mononucleotide adenylyltransferase
UniProt Gene Name:	NMNAT3
UniProt Entry Name:	NMNA3_HUMAN

	Western blot analysis of extracts of various cell lines, using NMNAT3 antibody (CAB14465) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using NMNAT3 Rabbit pAb (CAB14465) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using NMNAT3 Rabbit pAb (CAB14465) at dilution of 1:100. Blue: DAPI for nuclear staining.