Metabolism Antibodies 2

Anti-NMNAT1 Antibody (CAB6672)

(No reviews yet) Write a Review
SKU:
CAB6672
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-NMNAT1 Antibody
Antibody SKU:CAB6672
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NMNAT1 (NP_073624.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse liver, Mouse testis, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NMNAT1 (NP_073624.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MENS EKTE VVLL ACGS FNPI TNMH LRLF ELAK DYMN GTGR YTVV KGII SPVG DAYK KKGL IPAY HRVI MAEL ATKN SKWV EVDT WESL QKEW KETL KVLR HHQE KLEA SDCD HQQN SPTL ERPG RKRK WTET QDSS QKKS LEPK TKAV PK
Gene ID:64802
Uniprot:Q9HAN9
Cellular Location:Nucleus
Calculated MW:31kDa
Observed MW:32kDa
Synonyms:NMNAT1, LCA9, NMNAT, PNAT1
Background:This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15.
UniProt Protein Function:NMNAT1: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults. Belongs to the eukaryotic NMN adenylyltransferase family.
UniProt Protein Details:

Protein type:EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase; EC 2.7.7.1

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; ATP binding

Biological Process: NAD biosynthetic process; vitamin metabolic process; NAD metabolic process; water-soluble vitamin metabolic process

Disease: Leber Congenital Amaurosis 9

NCBI Summary:This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15. [provided by RefSeq, Jul 2014]
UniProt Code:Q9HAN9
NCBI GenInfo Identifier:30580491
NCBI Gene ID:64802
NCBI Accession:Q9HAN9.1
UniProt Secondary Accession:Q9HAN9,Q8TAE9, Q9H247, Q9H6B6, B1AN63,
UniProt Related Accession:Q9HAN9
Molecular Weight:279
NCBI Full Name:Nicotinamide mononucleotide adenylyltransferase 1
NCBI Synonym Full Names:nicotinamide nucleotide adenylyltransferase 1
NCBI Official Symbol:NMNAT1  
NCBI Official Synonym Symbols:LCA9; NMNAT; PNAT1  
NCBI Protein Information:nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1; pyridine nucleotide adenylyltransferase 1; nicotinate-nucleotide adenylyltransferase 1
UniProt Protein Name:Nicotinamide mononucleotide adenylyltransferase 1
UniProt Synonym Protein Names:Nicotinate-nucleotide adenylyltransferase 1 (EC:2.7.7.18); NaMN adenylyltransferase 1
UniProt Gene Name:NMNAT1  
UniProt Entry Name:NMNA1_HUMAN
Anti-NMNAT1 Antibody (CAB6672)Western blot analysis of extracts of various cell lines, using NMNAT1 antibody (CAB6672) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
View AllClose

0 Reviews

View AllClose

Related Products

Anti-NMNAT1 Antibody (CAB4937)

Anti-NMNAT1 Antibody (CAB4937)

Metabolism Antibodies 2

OverviewProduct Name:NMNAT1 Rabbit pAbProduct Code:CAB4937Size:20uL, 100uLSynonyms:NMNAT1, LCA9, NMNAT, PNAT1Applicati
Human NMNAT1/NMNAT Recombinant Protein (RPES1536)

Human NMNAT1/NMNAT Recombinant Protein (RPES1536)

Metabolism Recombinant Proteins

system_update_altDatasheetHuman NMNAT1/NMNAT Recombinant Protein NMNAT, also known as NMNAT1, is a member of the Nicotinamide-nucleotide adenylyltransferases. It is widely expressed with high l