Name: Anti-NME4 Antibody (CAB8350)
Brand: Cell Biology Antibodies 12
SKU: CAB8350
Availability: OutOfStock

Antibody Name:	Anti-NME4 Antibody
Antibody SKU:	CAB8350
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human NME4 (NP_005000.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, A-549, NCI-H460, BT-474, Mouse kidney, Mouse stomach, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human NME4 (NP_005000.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGGL FWRS ALRG LRCG PRAP GPSL LVRH GSGG PSWT RERT LVAV KPDG VQRR LVGD VIQR FERR GFTL VGMK MLQA PESV LAEH YQDL RRKP FYPA LIRY MSSG PVVA MVWE GYNV VRAS RAMI GHTD SAEA APGT IRGD FSVH ISRN VIHA SDSV EGAQ REIQ LWFQ SSEL VSWA DGGQ HSSI HPA
Gene ID:	4833
Uniprot:	O00746
Cellular Location:	Mitochondrion intermembrane space, Mitochondrion matrix, Peripheral membrane protein
Calculated MW:	12kDa/20kDa
Observed MW:	17kDa

Synonyms:	NME4, NDPK-D, NM23H4, nm23-H4
Background:

UniProt Protein Function:	NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family.
UniProt Protein Details:	Protein type:Kinase, nucleoside diphosphate; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; Kinase, other; Mitochondrial; Other group; NDK family Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrial inner membrane; mitochondrion Molecular Function:nucleoside diphosphate kinase activity; protein binding Biological Process: nucleobase, nucleoside and nucleotide interconversion; nucleoside metabolic process; nucleoside triphosphate biosynthetic process; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis
NCBI Summary:	The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]
UniProt Code:	O00746
NCBI GenInfo Identifier:	2498075
NCBI Gene ID:	4833
NCBI Accession:	O00746.1
UniProt Secondary Accession:	O00746,Q5U0M9, A2IDD0,
UniProt Related Accession:	O00746
Molecular Weight:	12,979 Da
NCBI Full Name:	Nucleoside diphosphate kinase, mitochondrial
NCBI Synonym Full Names:	NME/NM23 nucleoside diphosphate kinase 4
NCBI Official Symbol:	NME4
NCBI Official Synonym Symbols:	NDPK-D; NM23H4; nm23-H4
NCBI Protein Information:	nucleoside diphosphate kinase, mitochondrial
UniProt Protein Name:	Nucleoside diphosphate kinase, mitochondrial
UniProt Synonym Protein Names:	Nucleoside diphosphate kinase D; NDPKD; nm23-H4
UniProt Gene Name:	NME4
UniProt Entry Name:	NDKM_HUMAN

	Western blot analysis of extracts of various cell lines, using NME4 antibody (CAB8350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human liver using NME4 antibody (CAB8350) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using NME4 antibody (CAB8350) at dilution of 1:100 (40x lens).