Developmental Biology
Anti-NME1 Antibody (CAB0259)
- SKU:
- CAB0259
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-NME1 Antibody |
| Antibody SKU: | CAB0259 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 90 to the C-terminus of human NME1 (NP_000260.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, MCF7, HeLa, HepG2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 90 to the C-terminus of human NME1 (NP_000260.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLGE TNPA DSKP GTIR GDFC IQVG RNII HGSD SVES AEKE IGLW FHPE ELVD YTSC AQNW IYE |
| Gene ID: | 4830 |
| Uniprot: | P15531 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 17kDa/19kDa/30kDa |
| Observed MW: | 21kDa |
| Synonyms: | NME1, AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1 |
| Background: | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. |
| UniProt Protein Function: | NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported. |
| UniProt Protein Details: | Protein type:Kinase, other; EC 2.7.4.6; Tumor suppressor; Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Protein kinase, histidine; Nucleotide Metabolism - purine; Other group; NDK family Chromosomal Location of Human Ortholog: 17q21.3 Cellular Component: cytoplasm; cytosol; membrane; nucleus Molecular Function:ATP binding; deoxyribonuclease activity; GTP binding; identical protein binding; magnesium ion binding; nucleoside diphosphate kinase activity; protein binding; ribosomal small subunit binding Biological Process: negative regulation of cell proliferation; nucleobase, nucleoside and nucleotide interconversion; nucleoside triphosphate biosynthetic process; positive regulation of DNA binding; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis Disease: Neuroblastoma, Susceptibility To |
| NCBI Summary: | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P15531 |
| NCBI GenInfo Identifier: | 127981 |
| NCBI Gene ID: | 4830 |
| NCBI Accession: | P15531.1 |
| UniProt Secondary Accession: | P15531,Q6FGK3, Q86XQ2, Q9UDJ6, |
| UniProt Related Accession: | P15531 |
| Molecular Weight: | 30,137 Da |
| NCBI Full Name: | Nucleoside diphosphate kinase A |
| NCBI Synonym Full Names: | NME/NM23 nucleoside diphosphate kinase 1 |
| NCBI Official Symbol: | NME1 |
| NCBI Official Synonym Symbols: | NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1 |
| NCBI Protein Information: | nucleoside diphosphate kinase A |
| UniProt Protein Name: | Nucleoside diphosphate kinase A |
| UniProt Synonym Protein Names: | Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; NM23-H1; Tumor metastatic process-associated protein |
| Protein Family: | Nucleoside diphosphate kinase |
| UniProt Gene Name: | NME1 |
| UniProt Entry Name: | NDKA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NME1 antibody (CAB0259). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using NME1 Rabbit pAb (CAB0259) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat kidney using NME1 Rabbit pAb (CAB0259) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human colon using NME1 Rabbit pAb (CAB0259) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using NME1 antibody (CAB0259) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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