Name: Anti-NLRX1 Antibody (CAB18065)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18065
Availability: OutOfStock

Antibody Name:	Anti-NLRX1 Antibody [KO Validated]
Antibody SKU:	CAB18065
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 87-360 of human NLRX1 (NP_001269073.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 87-360 of human NLRX1 (NP_001269073.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AEWF SRLP REER QFGP TFAL DTVH VDPV IRES TPDE LLRP PAEL ALEH QPPQ AGLP PLAL SQLF NPDA CGRR VQTV VLYG TVGT GKST LVRK MVLD WCYG RLPA FELL IPFS CEDL SSLG PAPA SLCQ LVAQ RYTP LKEV LPLM AAAG SHLL FVLH GLEH LNLD FRLA GTGL CSDP EEPQ EPAA IIVN LLRK YMLP QASI LVTT RPSA IGRI PSKY VGRY GEIC GFSD TNLQ KLYF QLRL NQPY CGYA VGGS GVSA TPAQ RDHL VQML SRNL EG
Gene ID:	79671
Uniprot:	Q86UT6
Cellular Location:	Mitochondrion outer membrane
Calculated MW:	101kDa/107kDa
Observed MW:	108kDa

Anti-NLRX1 Antibody [KO Validated] (CAB18065)

Western blot analysis of extracts from normal (control) and NLRX1 knockout (KO) HeLa cells, using NLRX1 antibody (CAB18065) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Synonyms:	NLRX1, CLR11.3, DLNB26, NOD26, NOD5, NOD9
Background:	The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	NLRX1: Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF- Kappa-B and type 1 interferon signaling pathways, but enhances NF- Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). Interacts with MAVS. Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji, and Jurkat cell lines. Belongs to the NLRP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: mitochondrial outer membrane Molecular Function:protein binding; ATP binding Biological Process: viral reproduction; negative regulation of innate immune response; negative regulation of inflammatory response; negative regulation of interleukin-6 production; innate immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interferon type I production; negative regulation of interferon-beta production
NCBI Summary:	The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013]
UniProt Code:	Q86UT6
NCBI GenInfo Identifier:	74759406
NCBI Gene ID:	79671
NCBI Accession:	Q86UT6.1
UniProt Secondary Accession:	Q86UT6,Q7RTR3, Q96D51, Q9H724, A8K6Q1, B3KPK2, B3KTA2
UniProt Related Accession:	Q86UT6
Molecular Weight:	975
NCBI Full Name:	NLR family member X1
NCBI Synonym Full Names:	NLR family member X1
NCBI Official Symbol:	NLRX1
NCBI Official Synonym Symbols:	NOD5; NOD9; NOD26; DLNB26; CLR11.3
NCBI Protein Information:	NLR family member X1; NLR family, X1; NOD-like receptor X1; caterpiller protein 11.3; nucleotide-binding oligomerization domain protein 5; nucleotide-binding oligomerization domain protein 9; nucleotide-binding oligomerization domain protein 26; nucleotid
UniProt Protein Name:	NLR family member X1
UniProt Synonym Protein Names:	Caterpiller protein 11.3; CLR11.3; Nucleotide-binding oligomerization domain protein 26; Nucleotide-binding oligomerization domain protein 5; Nucleotide-binding oligomerization domain protein 9
Protein Family:	NLR family
UniProt Gene Name:	NLRX1
UniProt Entry Name:	NLRX1_HUMAN