Cell Biology Antibodies 3
Anti-NKX2-5 Antibody (CAB12688)
- SKU:
- CAB12688
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NKX2-5 Antibody |
| Antibody SKU: | CAB12688 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human NKX2-5 (NP_004378.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse heart, Rat heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human NKX2-5 (NP_004378.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AFKP EAYA GPEA AAPG LPEL RAEL GRAP SPAK CASA FPAA PAFY PRAY SDPD PAKD PRAE KKEL CALQ KAVE LEKT EADN AERP RARR RRKP RVLF SQAQ V |
| Gene ID: | 1482 |
| Uniprot: | P52952 |
| Cellular Location: | Nucleus |
| Calculated MW: | 11kDa/16kDa/34kDa |
| Observed MW: | 48kDa |
| Synonyms: | NKX2-5, CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3 |
| Background: | This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 5q34 Cellular Component: transcription factor complex; cytoplasm; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; chromatin binding; transcription factor binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; ventricular cardiac myofibril development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; atrial cardiac muscle cell development; positive regulation of cell proliferation; cardiac muscle morphogensis; thyroid gland development; hemopoiesis; regulation of cardiac muscle cell proliferation; heart looping; cell differentiation; vasculogenesis; cardiac muscle contraction; spleen development; adult heart development; pharyngeal system development; sarcomere organization; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; positive regulation of heart contraction; negative regulation of apoptosis Disease: Ventricular Septal Defect 3; Atrial Septal Defect 7 With Or Without Atrioventricular Conduction Defects; Conotruncal Heart Malformations; Tetralogy Of Fallot; Hypothyroidism, Congenital, Nongoitrous, 5; Hypoplastic Left Heart Syndrome 2 |
| NCBI Summary: | This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| UniProt Code: | P52952 |
| NCBI GenInfo Identifier: | 1708211 |
| NCBI Gene ID: | 1482 |
| NCBI Accession: | P52952.1 |
| UniProt Secondary Accession: | P52952,A8K3K0, B4DNB6, E9PBU6, |
| UniProt Related Accession: | P52952 |
| Molecular Weight: | 185 |
| NCBI Full Name: | Homeobox protein Nkx-2.5 |
| NCBI Synonym Full Names: | NK2 homeobox 5 |
| NCBI Official Symbol: | NKX2-5 |
| NCBI Official Synonym Symbols: | CSX; CSX1; VSD3; CHNG5; HLHS2; NKX2E; NKX2.5; NKX4-1 |
| NCBI Protein Information: | homeobox protein Nkx-2.5; tinman paralog; homeobox protein CSX; cardiac-specific homeobox 1; homeobox protein NK-2 homolog E; NK2 transcription factor related, locus 5 |
| UniProt Protein Name: | Homeobox protein Nkx-2.5 |
| UniProt Synonym Protein Names: | Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E |
| Protein Family: | Homeobox protein |
| UniProt Gene Name: | NKX2-5 |
| UniProt Entry Name: | NKX25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NKX2-5 antibody (CAB12688) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
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