Epigenetics & Nuclear Signaling Antibodies 3
Anti-NKX2-2 Antibody (CAB16696)
(No reviews yet)
Write a Review
- SKU:
- CAB16696
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-NKX2-2 Antibody |
| Antibody SKU: | CAB16696 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human NKX2-2 (NP_002500.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | mouse brain, C6, rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human NKX2-2 (NP_002500.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSLT NTKT GFSV KDIL DLPD TNDE EGSV AEGP EEEN EGPE PAKR AGPL GQGA LDAV QSLP LKNP FYDS SDNP YTRW LAST EGLQ YSLH GLAA GAPP QDSS SKSP EPSA DESP DNDK ETPG GGGD AGKK RK |
| Gene ID: | 4821 |
| Uniprot: | O95096 |
| Cellular Location: | |
| Calculated MW: | 30kDa |
| Observed MW: | 38kDa |
| Synonyms: | NKX2-2, NKX2.2, NKX2B |
| Background: | The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. |
| UniProt Protein Function: | NKX2-2: Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter. Belongs to the NK-2 homeobox family. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Transcription factor Chromosomal Location of Human Ortholog: 20p11.22 Cellular Component: nucleoplasm Molecular Function:chromatin binding; transcription coactivator activity Biological Process: brain development; endocrine pancreas development; positive regulation of transcription factor activity |
| NCBI Summary: | The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95096 |
| NCBI GenInfo Identifier: | 6016205 |
| NCBI Gene ID: | 4821 |
| NCBI Accession: | O95096.1 |
| UniProt Related Accession: | O95096 |
| Molecular Weight: | 30,133 Da |
| NCBI Full Name: | Homeobox protein Nkx-2.2 |
| NCBI Synonym Full Names: | NK2 homeobox 2 |
| NCBI Official Symbol: | NKX2-2 |
| NCBI Official Synonym Symbols: | NKX2B; NKX2.2 |
| NCBI Protein Information: | homeobox protein Nkx-2.2 |
| UniProt Protein Name: | Homeobox protein Nkx-2.2 |
| UniProt Synonym Protein Names: | Homeobox protein NK-2 homolog B |
| Protein Family: | Homeobox protein |
| UniProt Gene Name: | NKX2-2 |
| UniProt Entry Name: | NKX22_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NKX2-2 antibody (CAB16696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min. |
View AllClose
Related Products
NKX2-5 Polyclonal Antibody
OverviewProduct Name:NKX2-5 Polyclonal AntibodyProduct Code:CAB21992Reactivity:Mouse, RatApplications:Western blotting<