Signal Transduction Antibodies 1

Anti-NKAP Antibody (CAB13748)

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SKU:
CAB13748
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-NKAP Antibody
Antibody SKU:CAB13748
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human NKAP (NP_078804.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-431
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human NKAP (NP_078804.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAPV SGSR SPDR EASG SGGR RRSS SKSP KPSK SARS PRGR RSRS HSCS RSGD RNGL THQL GGLS QGSR NQSY RSRS RSRS RERP SAPR GIPF ASAS SSVY YGSY SRPY GSDK PWPS LLDK EREE SLRQ KR
Gene ID:79576
Uniprot:Q8N5F7
Cellular Location:Nucleus
Calculated MW:47kDa
Observed MW:47kDa
Synonyms:NKAP
Background:This gene encodes a protein that is involved in the activation of the ubiquitous transcription factor NF-kappaB. This protein is associated with the the histone deacetylase HDAC3 and with the Notch corepressor complex, and it thereby acts as a transcriptional repressor of Notch target genes. It is also required for alphabeta T cell development. A related pseudogene has been identified on chromosome X, while a related and intronless retrocopy, which has an intact CDS and may be functional, is located on chromosome 6.
UniProt Protein Function:NKAP: Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter. Belongs to the UPF0396 family.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: cytoplasm; nucleoplasm

Molecular Function:chromatin binding; protein binding

Biological Process: negative regulation of transcription, DNA-dependent; positive regulation of alpha-beta T cell differentiation

NCBI Summary:This gene encodes a protein that is involved in the activation of the ubiquitous transcription factor NF-kappaB. This protein is associated with the the histone deacetylase HDAC3 and with the Notch corepressor complex, and it thereby acts as a transcriptional repressor of Notch target genes. It is also required for alphabeta T cell development. A related pseudogene has been identified on chromosome X, while a related and intronless retrocopy, which has an intact CDS and may be functional, is located on chromosome 6. [provided by RefSeq, May 2010]
UniProt Code:Q8N5F7
NCBI GenInfo Identifier:74728990
NCBI Gene ID:79576
NCBI Accession:Q8N5F7.1
UniProt Secondary Accession:Q8N5F7,Q6IPW6, Q96BQ2, Q9H638,
UniProt Related Accession:Q8N5F7
Molecular Weight:47,138 Da
NCBI Full Name:NF-kappa-B-activating protein
NCBI Synonym Full Names:NFKB activating protein
NCBI Official Symbol:NKAP  
NCBI Protein Information:NF-kappa-B-activating protein
UniProt Protein Name:NF-kappa-B-activating protein
Protein Family:NKAP-like protein
UniProt Gene Name:NKAP  
UniProt Entry Name:NKAP_HUMAN
Anti-NKAP Antibody (CAB13748)Western blot analysis of extracts of A-431 cells, using NKAP antibody (CAB13748) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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