Cell Biology Antibodies 7

Anti-NIT1 Antibody (CAB16407)

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SKU:
CAB16407
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NIT1 Antibody
Antibody SKU:CAB16407
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NIT1 (NP_001172021.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NIT1 (NP_001172021.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLGF ITRP PHRF LSLL CPGL RIPQ LSVL CAQP RPRA MAIS SSSC ELPL VAVC QVTS TPDK QQNF KTCA ELVR EAAR LGAC LAFL PEAF DFIA RDPA ETLH LSEP LGGK LLEE YTQL AREC GLWL SLGG FHER GQDW EQTQ KIYN CHVL LN
Gene ID:4817
Uniprot:Q86X76
Cellular Location:Cytoplasm, Mitochondrion
Calculated MW:26kDa/31kDa/34kDa/35kDa/40kDa
Observed MW:34kDa
Synonyms:NIT1
Background:This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:NIT1: Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2. Belongs to the UPF0012 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.5.-.-; Hydrolase

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: nucleus

Molecular Function:nitrilase activity

NCBI Summary:This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:Q86X76
NCBI GenInfo Identifier:51704324
NCBI Gene ID:4817
NCBI Accession:Q86X76.2
UniProt Secondary Accession:Q86X76,O76091, B1AQP3, D3DVF4,
UniProt Related Accession:Q86X76
Molecular Weight:26,326 Da
NCBI Full Name:Nitrilase homolog 1
NCBI Synonym Full Names:nitrilase 1
NCBI Official Symbol:NIT1  
NCBI Protein Information:nitrilase homolog 1
UniProt Protein Name:Nitrilase homolog 1
Protein Family:Nitrilase
UniProt Gene Name:NIT1  
UniProt Entry Name:NIT1_HUMAN
Anti-NIT1 Antibody (CAB16407)Western blot analysis of extracts of rat liver, using NIT1 antibody (CAB16407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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