Antibody Name:	Anti-Nischarin Antibody
Antibody SKU:	CAB8935
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human NISCH (NP_009115.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, BT-474, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human NISCH (NP_009115.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLPQ PILS NQGI MFVQ EEAL ASSL SSTD SLTP EHQP IAQG CSDS LESI PAGQ AASD DLRD VPGA VGGA SPEH AEPE VQVV PGSG QIIF LPFT CIGY TATN QDFI QRLS TLIR QAIE RQLP AWIE AANQ REEG QGEQ GEEE DEEE EEEE DVAE NRYF EMGP PDVE EEEG GGQG EEEE EEEE DEEA EEER LALE WALG ADED F
Gene ID:	11188
Uniprot:	Q9Y2I1
Cellular Location:	Cell membrane, Cytoplasm, Early endosome, Recycling endosome
Calculated MW:	56kDa/63kDa/110kDa/166kDa
Observed MW:	166kDa

Synonyms:

NISCH, I-1, IR1, IRAS, hIRAS, nischarin

Background:

This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor.

UniProt Protein Function:	NISCH: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Receptor, GPCR; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21.1 Cellular Component: cytosol; membrane; plasma membrane Molecular Function:identical protein binding; protein binding
NCBI Summary:	This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]
UniProt Code:	Q9Y2I1
NCBI GenInfo Identifier:	296439287
NCBI Gene ID:	11188
NCBI Accession:	Q9Y2I1.3
UniProt Secondary Accession:	Q9Y2I1,Q6PGP3, Q6PIB4, Q7L8M3, Q7Z2X6, Q9UES6, Q9UEU4 Q9UFW3, C9J245,
UniProt Related Accession:	Q9Y2I1
Molecular Weight:	56,867 Da
NCBI Full Name:	Nischarin
NCBI Synonym Full Names:	nischarin
NCBI Official Symbol:	NISCH
NCBI Official Synonym Symbols:	I-1; IR1; IRAS; hIRAS
NCBI Protein Information:	nischarin
UniProt Protein Name:	Nischarin
UniProt Synonym Protein Names:	Imidazoline receptor 1; I-1; IR1; Imidazoline receptor antisera-selected protein; hIRAS; Imidazoline-1 receptor; I1R; Imidazoline-1 receptor candidate protein; I-1 receptor candidate protein; I1R candidate protein
Protein Family:	Nischarin
UniProt Gene Name:	NISCH
UniProt Entry Name:	NISCH_HUMAN