Cell Biology Antibodies 6
Anti-NIFK Antibody (CAB15595)
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- SKU:
- CAB15595
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-NIFK Antibody |
| Antibody SKU: | CAB15595 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-260 of human NIFK (NP_115766.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-260 of human NIFK (NP_115766.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GIDY DFPS LILQ KTES ISKT NRQT STKG QVLR KKKK KVSG TLDT PEKT VDSQ GPTP VCTP TFLE RRKS QVAE LNDD DKDD E |
| Gene ID: | 84365 |
| Uniprot: | Q9BYG3 |
| Cellular Location: | Chromosome, Nucleus, nucleolus |
| Calculated MW: | 34kDa |
| Observed MW: | 39kDa |
| Synonyms: | NIFK, MKI67IP, Nopp34 |
| Background: | This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19. |
| UniProt Protein Function: | NIFK: a nucleolar protein of unknown function that interacts with the fork-head associated (FHA) domain of the proliferation marker protein Ki-67 |
| UniProt Protein Details: | Protein type:Nucleolus; RNA-binding Chromosomal Location of Human Ortholog: 2q14.3 Cellular Component: condensed nuclear chromosome; cytoplasm; nucleolus; nucleoplasm Molecular Function:nucleotide binding; protein binding; RNA binding Biological Process: protein complex assembly; rRNA metabolic process; rRNA transcription |
| NCBI Summary: | This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009] |
| UniProt Code: | Q9BYG3 |
| NCBI GenInfo Identifier: | 71151919 |
| NCBI Gene ID: | 84365 |
| NCBI Accession: | Q9BYG3.1 |
| UniProt Secondary Accession: | Q9BYG3,Q8TB66, Q96ED4, A8K788, |
| UniProt Related Accession: | Q9BYG3 |
| Molecular Weight: | 34,222 Da |
| NCBI Full Name: | MKI67 FHA domain-interacting nucleolar phosphoprotein |
| NCBI Synonym Full Names: | nucleolar protein interacting with the FHA domain of MKI67 |
| NCBI Official Symbol: | NIFK |
| NCBI Official Synonym Symbols: | Nopp34; MKI67IP |
| NCBI Protein Information: | MKI67 FHA domain-interacting nucleolar phosphoprotein |
| UniProt Protein Name: | MKI67 FHA domain-interacting nucleolar phosphoprotein |
| UniProt Synonym Protein Names: | Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK |
| UniProt Gene Name: | NIFK |
| UniProt Entry Name: | MK67I_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NIFK antibody (CAB15595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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