Description
Antibody Name: | Anti-Niemann Pick C1 Antibody (CAB4795) |
Antibody SKU: | CAB4795 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthesized peptide derived from human Niemann Pick C1. |
Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | A-549, Mouse liver, Rat lung |
Immunogen: | A synthesized peptide derived from human Niemann Pick C1. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Late endosome membrane, Lysosome membrane, Multi-pass membrane protein |
Calculated MW: | 180kDa |
Observed MW: | 180KDa |
Synonyms: | NPC1, NPC, Niemann-Pick C1 protein |
Background: | This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments. |
Immunofluorescence analysis of HepG2 cells using Niemann Pick C1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of PC-12 cells using Niemann Pick C1 antibody at dilution of 1:50. Blue: DAPI for nuclear staining. | |
Immunohistochemistry of paraffin-embedded rat ovary using Niemann Pick C1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse liver using Niemann Pick C1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse kidney using Niemann Pick C1 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Western blot analysis of extracts of various cell lines, using Niemann Pick C1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. | |
Western blot analysis of extracts of Mouse liver, using Niemann Pick C1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |