Cell Biology Antibodies 12
Anti-Nidogen-1 Antibody (CAB8349)
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- SKU:
- CAB8349
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Nidogen-1 Antibody |
| Antibody SKU: | CAB8349 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-400 of human NID1 (NP_002499.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | LO2, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-400 of human NID1 (NP_002499.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DLDT TDGL GKVY YRED LSPS ITQR AAEC VHRG FPEI SFQP SSAV VVTW ESVA PYQG PSRD PDQK GKRN TFQA VLAS SDSS SYAI FLYP EDGL QFHT TFSK KENN QVPA VVAF SQGS VGFL WKSN GAYN IFAN DRES VENL AKSS NSGQ QGVW VFEI GSPA TTNG VVPA DVIL GTED GAEY DDED EDYD LATT RLGL EDVG TTPF SYKA LRRG GADT YSVP SVLS PRRA ATER PLGP PTER TRSF QLAV ETFH QQHP QVID VDEV EETG VVFS YNTD SRQT CANN RHQC SVH |
| Gene ID: | 4811 |
| Uniprot: | P14543 |
| Cellular Location: | Secreted, basement membrane, extracellular matrix, extracellular space |
| Calculated MW: | 122kDa/136kDa |
| Observed MW: | 178kDa |
| Synonyms: | NID1, NID, nidogen-1 |
| Background: | This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. |
| UniProt Protein Function: | Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 1q43 Cellular Component: extracellular matrix; extracellular region; basement membrane; basal lamina Molecular Function:laminin-1 binding; collagen binding; proteoglycan binding; laminin binding; calcium ion binding Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell-matrix adhesion; glomerular basement membrane development |
| NCBI Summary: | This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14543 |
| NCBI GenInfo Identifier: | 251757450 |
| NCBI Gene ID: | 4811 |
| NCBI Accession: | P14543.3 |
| UniProt Related Accession: | P14543 |
| Molecular Weight: | |
| NCBI Full Name: | Nidogen-1 |
| NCBI Synonym Full Names: | nidogen 1 |
| NCBI Official Symbol: | NID1 |
| NCBI Official Synonym Symbols: | NID |
| NCBI Protein Information: | nidogen-1 |
| UniProt Protein Name: | Nidogen-1 |
| UniProt Synonym Protein Names: | Entactin |
| Protein Family: | Nidogen |
| UniProt Gene Name: | NID1 |
| UniProt Entry Name: | NID1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NID1 antibody (CAB8349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human stomach using NID1 antibody (CAB8349) at dilution of 1:100 (40x lens). |
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