Signal Transduction Antibodies 2

Anti-NICN1 Antibody (CAB15532)

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SKU:
CAB15532
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-NICN1 Antibody
Antibody SKU:CAB15532
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human NICN1 (NP_115692.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human NICN1 (NP_115692.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSRV LVPC HVKG SVAL QVGD VRTS QGRP GVLV IDVT FPSV APFE LQEI TFKN YYTA FLSI RVRQ YTSA HTPA KWVT CLRD YCLM PDPH SEEG AQEY VSLF KHQM LCDM ARIS ELRL ILRQ PSPL WLSF TVEE LQIY QQGP KSPS VTFP KWLS HPVP CEQP ALLR EGLP DPSR VSSE VQQM WALT EMIR ASHT SARI GRFD VDGC YDLN LLSY T
Gene ID:84276
Uniprot:Q9BSH3
Cellular Location:Nucleus
Calculated MW:19kDa/24kDa
Observed MW:18kDa
Synonyms:NICN1, nicolin-1
Background:This protein encoded by this gene localizes to the nucleus and is expressed in numerous tissues including brain, testis, liver, and kidney. This refseq contains genomic sequence in its 3' UTR which is not supported by experimental evidence. Computer predictions indicate that this region of the 3' UTR contains hairpin-forming self-complementary sequence which is possibly excised after transcription. This gene has a pseudogene on chromosome X.
UniProt Protein Function:NICN1: 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: microtubule; nucleus

NCBI Summary:This protein encoded by this gene localizes to the nucleus and is expressed in numerous tissues including brain, testis, liver, and kidney. This refseq contains genomic sequence in its 3' UTR which is not supported by experimental evidence. Computer predictions indicate that this region of the 3' UTR contains hairpin-forming self-complementary sequence which is possibly excised after transcription. This gene has a pseudogene on chromosome X. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BSH3
NCBI GenInfo Identifier:14150084
NCBI Gene ID:84276
NCBI Accession:NP_115692.1
UniProt Secondary Accession:Q9BSH3,Q8IZQ2,
UniProt Related Accession:Q9BSH3
Molecular Weight:24,202 Da
NCBI Full Name:nicolin-1
NCBI Synonym Full Names:nicolin 1
NCBI Official Symbol:NICN1  
NCBI Protein Information:nicolin-1; PGs5; NPCEDRG; tubulin polyglutamylase complex subunit 5
UniProt Protein Name:Nicolin-1
UniProt Synonym Protein Names:NPCEDRG; Tubulin polyglutamylase complex subunit 5
Protein Family:Nicolin
UniProt Gene Name:NICN1  
UniProt Entry Name:NICN1_HUMAN
Anti-NICN1 Antibody (CAB15532)Western blot analysis of extracts of various cell lines, using NICN1 antibody (CAB15532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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