Anti-NG2/CSPG4 Antibody (CAB21095)

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SKU:
CAB21095

Description

Antibody Name:Anti-NG2/CSPG4 Antibody (CAB21095)
Antibody SKU:CAB21095
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LGAP LEGV LVEL EVLP AAIP LEAQ NFSV PEGG SLTL APPL LRVS GPYF PTLL GLSL QVLE PPQH GALQ KEDG PQAR TLSA FSWR MVEE QLIR YVHD GSET LTDS FVLM ANAS EMDR QSHP VAFT VTVL PVND QPPI LTTN TGLQ MWEG ATAP IPAE ALRS TDGD SGSE DLVY TIEQ PSNG RVVL RGAP GTEV RSFT QAQL DGGL VLFS HRGT LDGG FRFR LSDG
Cellular Location:Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane
Calculated MW:250kDa
Observed MW:Refer to figures
Synonyms:CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
Background:A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Western blot analysis of extracts of A375 cells, using NG2/CSPG4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.Western blot analysis of extracts of A375 cells, using NG2/CSPG4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.
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Additional Information

Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Universal
Host Species:
Rabbit
Isotype:
IgG
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