Anti-NG2/CSPG4 Antibody (CAB21095)
- SKU:
- CAB21095
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Universal
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-NG2/CSPG4 Antibody (CAB21095) |
| Antibody SKU: | CAB21095 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LGAP LEGV LVEL EVLP AAIP LEAQ NFSV PEGG SLTL APPL LRVS GPYF PTLL GLSL QVLE PPQH GALQ KEDG PQAR TLSA FSWR MVEE QLIR YVHD GSET LTDS FVLM ANAS EMDR QSHP VAFT VTVL PVND QPPI LTTN TGLQ MWEG ATAP IPAE ALRS TDGD SGSE DLVY TIEQ PSNG RVVL RGAP GTEV RSFT QAQL DGGL VLFS HRGT LDGG FRFR LSDG |
| Cellular Location: | Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane |
| Calculated MW: | 250kDa |
| Observed MW: | Refer to figures |
| Synonyms: | CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2 |
| Background: | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. |
 | Western blot analysis of extracts of A375 cells, using NG2/CSPG4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S. |
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