Cell Biology Antibodies 8

Anti-NFYA Antibody (CAB1998)

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SKU:
CAB1998
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NFYA Antibody
Antibody SKU:CAB1998
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human NFYA (NP_068351.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human
Positive Samples:HeLa, LO2, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human NFYA (NP_068351.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEQY TANS NSST EQIV VQAG QIQQ QVQG QPLM VQVS GGQL ITST GQPI MVQA VPGG QGQT IMQV PVSG TQGL QQIQ LVPP GQIQ IQGG QAVQ VQGQ QGQT QQII IQQP QTAV TAGQ TQTQ QQIA VQGQ QVAQ TAEG QTIV YQPV NADG TILQ QVTV PVSG MITI PAAS LAGA QIVQ TGAN TNTT SSGQ GTVT VTLP VAGN VVNS GGMV MMVP GAGS VPAI QRIP LPGA EMLE EEPL YVNA KQYH RILK RRQA RAKL EAEG KIPK ERRK YLHE SRHR HAMA RKRG EGGR FFSP KEKD SPHM QDPN QADE EAMT QIIR VS
Gene ID:4800
Uniprot:P23511
Cellular Location:Nucleus
Calculated MW:33kDa/36kDa
Observed MW:37kDa
Synonyms:NFYA, CBF-A, CBF-B, HAP2, NF-YA
Background:The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
UniProt Protein Function:NFYA: stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. Involved in cisplatin resistance. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: CCAAT-binding factor complex; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter

NCBI Summary:The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:P23511
NCBI GenInfo Identifier:115844
NCBI Gene ID:4800
NCBI Accession:P23511.2
UniProt Secondary Accession:P23511,Q8IXU0,
UniProt Related Accession:P23511
Molecular Weight:37kDa
NCBI Full Name:Nuclear transcription factor Y subunit alpha
NCBI Synonym Full Names:nuclear transcription factor Y subunit alpha
NCBI Official Symbol:NFYA  
NCBI Official Synonym Symbols:HAP2; CBF-A; CBF-B; NF-YA  
NCBI Protein Information:nuclear transcription factor Y subunit alpha
UniProt Protein Name:Nuclear transcription factor Y subunit alpha
UniProt Synonym Protein Names:CAAT box DNA-binding protein subunit A; Nuclear transcription factor Y subunit A; NF-YA
Protein Family:Nuclear transcription factor
UniProt Gene Name:NFYA  
Anti-NFYA Antibody (CAB1998)Western blot analysis of extracts of various cell lines, using NFYA antibody (CAB1998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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