Epigenetics & Nuclear Signaling Antibodies 1

Anti-NFKBIZ Antibody (CAB10492)

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SKU:
CAB10492
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NFKBIZ Antibody
Antibody SKU:CAB10492
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFKBIZ (NP_113607.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, THP-1, Mouse kidney, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFKBIZ (NP_113607.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MIVD KLLD DSRG GEGL RDAA GGCG LMTS PLNL SYFY GASP PAAA PGAC DASC SVLG PSAP GSPG SDSS DFSS ASSV SSCG AVES RSRG GARA ERQP VEPH MGVG RQQR GPFQ GVRV KNSV KELL LHIR SHKQ KASG QAVD DFKT QGVN IEQF RELK NTVS YSGK RKGP DSLS DGPA CKRP ALLH SQFL TPPQ TPTP GESM EDVH LNEP KQES SADL LQNI
Gene ID:64332
Uniprot:Q9BYH8
Cellular Location:Nucleus
Calculated MW:64kDa/68kDa/78kDa
Observed MW:68-90kDa
Synonyms:NFKBIZ, IKBZ, INAP, MAIL
Background:This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:IkB-zeta: Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF- kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis. Interacts with NFKB1/p50. Interacts with RELA. By TNF, IL1/interleukin-1 and bacterial lipopolysaccharides (LPS). Expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 3q12.3

Cellular Component: nuclear speck; nucleus

Molecular Function:protein binding; transcription cofactor activity

Biological Process: regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BYH8
NCBI GenInfo Identifier:74752456
NCBI Gene ID:64332
NCBI Accession:Q9BYH8.1
UniProt Secondary Accession:Q9BYH8,Q8IUL4, Q8NAZ8, B3KNR2, D3DN54,
UniProt Related Accession:Q9BYH8
Molecular Weight:78kDa
NCBI Full Name:NF-kappa-B inhibitor zeta
NCBI Synonym Full Names:NFKB inhibitor zeta
NCBI Official Symbol:NFKBIZ  
NCBI Official Synonym Symbols:IKBZ; INAP; MAIL  
NCBI Protein Information:NF-kappa-B inhibitor zeta
UniProt Protein Name:NF-kappa-B inhibitor zeta
UniProt Synonym Protein Names:I-kappa-B-zeta; IkB-zeta; IkappaBzeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; Molecule possessing ankyrin repeats induced by lipopolysaccharide; MAIL
Protein Family:NF-kappa-B inhibitor
UniProt Gene Name:NFKBIZ  
Anti-NFKBIZ Antibody (CAB10492)Western blot analysis of extracts of various cell lines, using NFKBIZ antibody (CAB10492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-NFKBIZ Antibody (CAB10492)Immunofluorescence analysis of Raw264.7 cells using NFKBIZ antibody (CAB10492) at dilution of 1:100. Blue: DAPI for nuclear staining.
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