Cell Biology Antibodies 12

Anti-NFKBIE Antibody (CAB8443)

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SKU:
CAB8443
Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NFKBIE Antibody
Antibody SKU:CAB8443
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human NFKBIE (NP_004547.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse liver, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human NFKBIE (NP_004547.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSEA RKGP DEAE ESQY DSGI ESLR SLRS LPES TSAP ASGP SDGS PQPC THPP GPVK EPQE KEDA DGER ADST YGSS SLTY TLSL LGGP EAED PAPR LPLP HVGA LSPQ QLEA LTYI SEDG DTLV HLAV IHEA PAVL LCCL A
Gene ID:4794
Uniprot:O00221
Cellular Location:Cytoplasm
Calculated MW:52kDa
Observed MW:60kDa
Synonyms:NFKBIE, IKBE
Background:The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus.
UniProt Protein Function:IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family.
UniProt Protein Details:

Protein type:DNA-binding; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytoplasm; cytosol

Molecular Function:protein binding

Biological Process: cytoplasmic sequestering of transcription factor

NCBI Summary:The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]
UniProt Code:O00221
NCBI GenInfo Identifier:206729919
NCBI Gene ID:4794
NCBI Accession:O00221.3
UniProt Secondary Accession:O00221,Q5T9V9,
UniProt Related Accession:O00221
Molecular Weight:52,864 Da
NCBI Full Name:NF-kappa-B inhibitor epsilon
NCBI Synonym Full Names:NFKB inhibitor epsilon
NCBI Official Symbol:NFKBIE  
NCBI Official Synonym Symbols:IKBE  
NCBI Protein Information:NF-kappa-B inhibitor epsilon
UniProt Protein Name:NF-kappa-B inhibitor epsilon
UniProt Synonym Protein Names:I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon
Protein Family:NF-kappa-B inhibitor
UniProt Gene Name:NFKBIE  
UniProt Entry Name:IKBE_HUMAN
Anti-NFKBIE Polyclonal Antibody (CAB8443)Western blot analysis of extracts of various cell lines, using NFKBIE antibody (CAB8443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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