Cell Biology Antibodies 9

Anti-NFKBIB Antibody (CAB5777)

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SKU:
CAB5777
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-NFKBIB Antibody
Antibody SKU:CAB5777
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-356 of human NFKBIB (NP_002494.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, 293T, Mouse liver, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-356 of human NFKBIB (NP_002494.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGV ACLG KAAD ADEW CDSG LGSL GPDA AAPG GPGL GAEL GPGL SWAP LVFG YVTE DGDT ALHL AVIH QHEP FLDF LLGF SAGT EYMD LQND LGQT ALHL AAIL GETS TVEK LYAA GAGL CVAE RRGH TALH LACR VGAH ACAR ALLQ PRPR RPRE APDT YLAQ GPDR TPDT NHTP VALY PDSD LEKE EEES EEDW KLQL EAEN YEGH TPLH VAVI HKDV EMVR LLRD AGAD LDKP EPTC GRSP LHLA VEAQ AADV LELL LRAG ANPA ARMY GGRT PLGS AMLR PNPI LARL LRAH GAPE PEGE DEKS GPCS SSSD SDSG DEGD EYDD IVVH SSRS QTRL PPTP ASKP LPDD PRPV
Gene ID:4793
Uniprot:Q15653
Cellular Location:Cytoplasm, Nucleus
Calculated MW:35kDa/37kDa
Observed MW:40-50kDa
Synonyms:NFKBIB, IKBB, TRIP9
Background:The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:IkB-beta: a protein of the NF-kappa-B inhibitor family. Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA- dependent inactivation. Association with inhibitor kappa B- interacting Ras-like proteins 1 and 2 may also explain the slower degradation. Interacts with ligand-binding domain of thyroid hormone receptor-beta, inhibitor kappa B-interacting Ras-like protein 1, inhibitor kappa B-interacting protein Ras2, p65 (RELA) and c-Rel. Expressed in all tissues examined.
UniProt Protein Details:

Protein type:Inhibitor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytosol; nucleus

Molecular Function:protein binding; signal transducer activity; transcription coactivator activity

Biological Process: transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

NCBI Summary:The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]
UniProt Code:Q15653
NCBI GenInfo Identifier:57015399
NCBI Gene ID:4793
NCBI Accession:Q15653.2
UniProt Secondary Accession:Q15653,Q96BJ7, A8K3F4,
UniProt Related Accession:Q15653
Molecular Weight:356
NCBI Full Name:NF-kappa-B inhibitor beta
NCBI Synonym Full Names:nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
NCBI Official Symbol:NFKBIB  
NCBI Official Synonym Symbols:IKBB; TRIP9  
NCBI Protein Information:NF-kappa-B inhibitor beta; ikB-B; TRIP-9; ikB-beta; ikappaBbeta; NF-kappa-BIB; I-kappa-B-beta; TR-interacting protein 9; thyroid receptor-interacting protein 9
UniProt Protein Name:NF-kappa-B inhibitor beta
UniProt Synonym Protein Names:I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9
Protein Family:NF-kappa-B inhibitor
UniProt Gene Name:NFKBIB  
UniProt Entry Name:IKBB_HUMAN
Anti-NFKBIB Antibody (CAB5777)Western blot analysis of extracts of various cell lines, using NFKBIB antibody (CAB5777) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
Anti-NFKBIB Antibody (CAB5777)Immunohistochemistry of paraffin-embedded rat lung using NFKBIB antibody (CAB5777) at dilution of 1:200 (40x lens).
Anti-NFKBIB Antibody (CAB5777)Immunohistochemistry of paraffin-embedded human liver cancer using NFKBIB antibody (CAB5777) at dilution of 1:200 (40x lens).
Anti-NFKBIB Antibody (CAB5777)Immunohistochemistry of paraffin-embedded human kidney cancer using NFKBIB antibody (CAB5777) at dilution of 1:200 (40x lens).
Anti-NFKBIB Antibody (CAB5777)Immunofluorescence analysis of U2OS cells using NFKBIB antibody (CAB5777). Blue: DAPI for nuclear staining.
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