Epigenetics & Nuclear Signaling Antibodies 4

Anti-NFIX Antibody (CAB9390)

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SKU:
CAB9390
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NFIX Antibody
Antibody SKU:CAB9390
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-260 of human NFIX (NP_002492.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human, Mouse
Positive Samples:A-431, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-260 of human NFIX (NP_002492.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AYFV HTPE SGQS DSSN QQGD ADIK PLPN GHLS FQDC FVTS GVWN VTEL VRVS QTPV ATAS GPNF SLAD LESP SYYN INQV T
Gene ID:4784
Uniprot:Q14938
Cellular Location:Nucleus
Calculated MW:47kDa/48kDa/50kDa/54kDa/55kDa
Observed MW:50kDa
Synonyms:NFIX, CTF, MRSHSS, NF-I/X, NF1-X, NF1A, SOTOS2
Background:The protein encoded by this gene is a transcription factor that binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3 in viral and cellular promoters. The encoded protein can also stimulate adenovirus replication in vitro. Three transcript variants encoding different isoforms have been found for this gene.
NCBI Summary:The protein encoded by this gene is a transcription factor that binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3 in viral and cellular promoters. The encoded protein can also stimulate adenovirus replication in vitro. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]
UniProt Code:Q14938
NCBI GenInfo Identifier:56549649
NCBI Gene ID:4784
NCBI Accession:NP_002492
UniProt Related Accession:Q14938
Molecular Weight:49kDa
NCBI Full Name:nuclear factor 1 X-type isoform 2
NCBI Synonym Full Names:nuclear factor I X
NCBI Official Symbol:NFIX  
NCBI Official Synonym Symbols:CTF; NF1A; NF1-X; MRSHSS; NF-I/X; SOTOS2  
NCBI Protein Information:nuclear factor 1 X-type
UniProt Protein Name:Nuclear factor 1 X-type
UniProt Synonym Protein Names:CCAAT-box-binding transcription factor; CTF; Nuclear factor I/X; NF-I/X; NFI-X; TGGCA-binding protein
Protein Family:Nuclear factor
UniProt Gene Name:NFIX  
UniProt Entry Name:NFIX_HUMAN
Anti-NFIX Polyclonal Antibody (CAB9390)Western blot analysis of extracts of various cell lines, using NFIX antibody (CAB9390) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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