Cell Biology Antibodies 4
Anti-NFIL-3 Antibody (CAB14029)
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- SKU:
- CAB14029
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | NFIL3 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB14029 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 133-462 of human NFIL3 (NP_005375.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IF 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 133-462 of human NFIL3 (NP_005375.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AYAQ EIQK LSNS TAVY FQDY QTSK SNVS SFVD EHEP SMVS SSCI SVIK HSPQ SSLS DVSE VSSV EHTQ ESSV QGSC RSPE NKFQ IIKQ EPME LESY TREP RDDR GSYT ASIY QNYM GNSF SGYS HSPP LLQV NRSS SNSP RTSE TDDG VVGK SSDG EDEQ QVPK GPIH SPVE LKHV HATV VKVP EVNS SALP HKLR IKAK AMQI KVEA FDNE FEAT QKLS SPID MTSK RHFE LEKH SAPS MVHS SLTP FSVQ VTNI QDWS LKSE HWHQ KELS GKTQ NSFK TGVV EMKD SGYK VSDP ENLY LKQG IANL SAEV VSLK RLIA TQPI SASD SG |
| Gene ID: | 4783 |
| Uniprot: | Q16649 |
| Cellular Location: | Nucleus |
| Calculated MW: | 51kDa |
| Observed MW: | 55kDa |
| Synonyms: | NFIL3, E4BP4, IL3BP1, NF-IL3A, NFIL3A |
| Background: | The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | E4BP4: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B- site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Belongs to the bZIP family. NFIL3 subfamily. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 9q22 Cellular Component: nucleus Molecular Function:DNA binding; transcription corepressor activity; transcription factor activity Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; immune response; negative regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014] |
| UniProt Code: | Q16649 |
| NCBI GenInfo Identifier: | 150385077 |
| NCBI Gene ID: | 4783 |
| NCBI Accession: | Q16649.2 |
| UniProt Secondary Accession: | Q16649,Q14211, Q6FGQ8, Q96HS0, B2R9Y8, |
| UniProt Related Accession: | Q16649 |
| Molecular Weight: | Calculated MW:51kDaObserved MW: 55kDa |
| NCBI Full Name: | Nuclear factor interleukin-3-regulated protein |
| NCBI Synonym Full Names: | nuclear factor, interleukin 3 regulated |
| NCBI Official Symbol: | NFIL3 |
| NCBI Official Synonym Symbols: | E4BP4; IL3BP1; NFIL3A; NF-IL3A |
| NCBI Protein Information: | nuclear factor interleukin-3-regulated protein; E4 promoter-binding protein 4; interleukin-3-binding protein 1; transcriptional activator NF-IL3A; nuclear factor interleukin 3 regulated protein; interleukin-3 promoter transcriptional activator |
| UniProt Protein Name: | Nuclear factor interleukin-3-regulated protein |
| UniProt Synonym Protein Names: | E4 promoter-binding protein 4; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; Transcriptional activator NF-IL3A |
| Protein Family: | Nuclear factor interleukin-3-regulated protein |
| UniProt Gene Name: | NFIL3 |
| UniProt Entry Name: | NFIL3_HUMAN |
 | Western blot analysis of extracts of mouse liver, using NFIL3 antibody (CAB14029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody (CAB14029). Blue: DAPI for nuclear staining. |
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