Epigenetics & Nuclear Signaling Antibodies 2
Anti-NFIC Antibody (CAB15074)
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- SKU:
- CAB15074
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-NFIC Antibody |
| Antibody SKU: | CAB15074 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFIC (NP_005588.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, LO2, MCF-7, DU145, NIH/3T3, mouse liver, mouse skeletal muscle, rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFIC (NP_005588.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MYSS PLCL TQDE FHPF IEAL LPHV RAFA YTWF NLQA RKRK YFKK HEKR MSKD EERA VKDE LLGE KPEV KQKW ASRL LAKL RKDI RPEC REDF VLSI TGKK APGC VLSN PDQK GKMR RIDC LRQA DKVW RLDL VMVI LFKG IPLE STDG ERLV KAAQ CGHP VLCV QPHH IGVA VKEL DLYL AYFV RERD AEQS GSPR TGMG SDQE DSKP ITLD TTDF QESF |
| Gene ID: | 4782 |
| Uniprot: | P08651 |
| Cellular Location: | Nucleus |
| Calculated MW: | 45kDa/47kDa/48kDa/54kDa/55kDa |
| Observed MW: | 60kDa |
| Synonyms: | NFIC, CTF, CTF5, NF-I, NFI |
| Background: | The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| UniProt Protein Function: | NFI-C: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleolus; nucleus Molecular Function:transcription factor activity Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P08651 |
| NCBI GenInfo Identifier: | 14195672 |
| NCBI Gene ID: | 4782 |
| NCBI Accession: | P08651.2 |
| UniProt Secondary Accession: | P08651,P08652, Q14932, Q9UPJ3, Q9UPJ9, Q9UPK0, Q9UPK1 A8K1H0, B7Z4U5, B7Z9C3, K7EMU1, |
| UniProt Related Accession: | P08651 |
| Molecular Weight: | 48,894 Da |
| NCBI Full Name: | Nuclear factor 1 C-type |
| NCBI Synonym Full Names: | nuclear factor I C |
| NCBI Official Symbol: | NFIC |
| NCBI Official Synonym Symbols: | CTF; NFI; CTF5; NF-I |
| NCBI Protein Information: | nuclear factor 1 C-type |
| UniProt Protein Name: | Nuclear factor 1 C-type |
| UniProt Synonym Protein Names: | CCAAT-box-binding transcription factor; CTF; Nuclear factor I/C; NF-I/C; NFI-C; TGGCA-binding protein |
| Protein Family: | Nuclear factor |
| UniProt Gene Name: | NFIC |
| UniProt Entry Name: | NFIC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NFIC antibody (CAB15074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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