Epigenetics & Nuclear Signaling Antibodies 2

Anti-NFIB Antibody (CAB15294)

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SKU:
CAB15294
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-NFIB Antibody
Antibody SKU:CAB15294
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human NFIB (NP_005587.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human NFIB (NP_005587.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SRTW HERD QDMS SPTT MKKP EKPL FSSA SPQD SSPR LSTF PQHH HPGI PGVA HSVI STRT PPPP SPLP FPTQ AILP PAPS SYFS HPTI RYPP HLNP QDTL KNYV PSYD PSSP QTSQ SWYL G
Gene ID:4781
Uniprot:O00712
Cellular Location:Nucleus
Calculated MW:22kDa/33kDa/47kDa/53kDa/55kDa
Observed MW:47kDa
Synonyms:NFIB, CTF, HMGIC/NFIB, NF-I/B, NF1-B, NFI-B, NFI-RED, NFIB2, NFIB3
Background:
UniProt Protein Function:NFI-B: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function:DNA binding; double-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; pontine nucleus development; glial cell differentiation; anterior commissure morphogenesis; chondrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; DNA replication; negative regulation of DNA binding; hindbrain development

UniProt Code:O00712
NCBI GenInfo Identifier:45644934
NCBI Gene ID:4781
NCBI Accession:O00712.2
UniProt Secondary Accession:O00712,O00166, Q12858, Q5VW29, Q63HM5, Q6ZNF9, Q96J45 G3V1P1, H7BYE8,
UniProt Related Accession:O00712
Molecular Weight:420
NCBI Full Name:Nuclear factor 1 B-type
NCBI Synonym Full Names:nuclear factor I/B
NCBI Official Symbol:NFIB  
NCBI Official Synonym Symbols:CTF; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB  
NCBI Protein Information:nuclear factor 1 B-type; nuclear factor 1/B; TGGCA-binding protein; CCAAT-box-binding transcription factor
UniProt Protein Name:Nuclear factor 1 B-type
UniProt Synonym Protein Names:CCAAT-box-binding transcription factor; CTF; Nuclear factor I/B; NF-I/B; NFI-B; TGGCA-binding protein
Protein Family:Nuclear factor
UniProt Gene Name:NFIB  
UniProt Entry Name:NFIB_HUMAN
Anti-NFIB Antibody (CAB15294)Western blot analysis of extracts of HeLa cells, using NFIB antibody (CAB15294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
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